Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis

Autor(es):Mostrar menos -

Maria Marcos, Caroline ^[1] ; de Fatima da Silva, Julhiany ^[1] ; Cesar de Oliveira, Haroldo ^[1] ; Akemi Assato, Patricia ^[1] ; de Lacorte Singulani, Junya ^[1] ; Maria Lopez, Angela ^[2] ; Patricia Tamayo, Diana ^[2] ; Hernandez-Ruiz, Orville ^{[3, 2]} ; McEwen, Juan G. ^{[4, 2]} ; Soares Mendes-Giannini, Maria Jose ^[1] ; Marisa Fusco-Almeida, Ana ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Analises Clin, Lab Micol Clin, Araraquara, SP - Brazil

^[2] CIB, Unidad Biol Celular Mol, Medellin - Colombia

^[3] Univ Antioquia, Escuela Microbiol, Medellin - Colombia

^[4] Univ Antioquia, Fac Med, Medellin - Colombia

• Tipo de documento: Artigo Científico
• Fonte: VIRULENCE; v. 7, n. 2, p. 72-84, FEB 17 2016.
• Citações Web of Science: 9

Resumo

The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by similar to 55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis. (AU)

• Processo FAPESP: 15/03700-9 - Animais alternativos aplicados à interação Paracoccidioides-hospedeiro para o desenvolvimento de estudos de virulência, eficácia e toxicidade de compostos antifúngicos e tratamentos preventivos
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/18038-9 - Caracterização e análise funcional do gene da proteína 14-3-3 envolvida no processo de adesão do complexo Paracoccidioides sp. e identificação de alvos potenciais na terapia da paracoccidioidomicose
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular