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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Decreased expression of 14-3-3 in Paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis

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Author(s):
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Maria Marcos, Caroline [1] ; de Fatima da Silva, Julhiany [1] ; Cesar de Oliveira, Haroldo [1] ; Akemi Assato, Patricia [1] ; de Lacorte Singulani, Junya [1] ; Maria Lopez, Angela [2] ; Patricia Tamayo, Diana [2] ; Hernandez-Ruiz, Orville [3, 2] ; McEwen, Juan G. [4, 2] ; Soares Mendes-Giannini, Maria Jose [1] ; Marisa Fusco-Almeida, Ana [1]
Total Authors: 11
Affiliation:
[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Analises Clin, Lab Micol Clin, Araraquara, SP - Brazil
[2] CIB, Unidad Biol Celular Mol, Medellin - Colombia
[3] Univ Antioquia, Escuela Microbiol, Medellin - Colombia
[4] Univ Antioquia, Fac Med, Medellin - Colombia
Total Affiliations: 4
Document type: Journal article
Source: VIRULENCE; v. 7, n. 2, p. 72-84, FEB 17 2016.
Web of Science Citations: 9
Abstract

The interaction between the fungal pathogen Paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. One molecule implicated in the P. brasiliensis-host interaction is the 14-3-3 protein. The 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. Here, we investigated the relevance of the 14-3-3 protein to the virulence of P. brasiliensis. Using antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated a 14-3-3-silenced strain (expression reduced by similar to 55%). This strain allowed us to investigate the interaction between 14-3-3 and the host and to correlate the functions of P. brasiliensis 14-3-3 with cellular features, such as morphological characteristics and virulence, that are important for pathogenesis. (AU)

FAPESP's process: 15/03700-9 - Alternative animal as a model to study Paracoccidioides- host interaction: virulence, efficacy and toxicology of antifungal compounds and new preventive treatments
Grantee:Maria José Soares Mendes Giannini
Support Opportunities: Regular Research Grants
FAPESP's process: 11/18038-9 - Characterization and functional analysis of the gene for 14-3-3 protein involved in the process of adhesion of Paracoccidioides sp. complex and identification of potential targets in the treatment of paracoccidioidomycosis
Grantee:Maria José Soares Mendes Giannini
Support Opportunities: Regular Research Grants