Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection

Autor(es)

Ramos-Gonzalez, Pedro Luis ^[1] ; Sarubbi-Orue, Humberto ^[2] ; Gonzales-Segnana, Luis ^[2] ; Chabi-Jesus, Camila ^{[3, 1]} ; Freitas-Astua, Juliana ^{[4, 1]} ; Kitajima, Eliot Watanabe ^[3]

Afiliação do(s) autor(es)

^[1] IAC, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP - Brazil

^[2] Univ Nacl Asuncion, Asuncion 1618 - Paraguay

^[3] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Piracicaba, SP - Brazil

^[4] Embrapa Cassava & Fruits, Cruz Das Almas, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYTOPATHOLOGY; v. 164, n. 5, p. 342-347, MAY 2016.
• Citações Web of Science: 7

Resumo

The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. (AU)

• Processo FAPESP: 08/57909-2 - Plataforma genômica aplicada ao melhoramento de citros
• Beneficiário: Marcos Antonio Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/08458-9 - Vírus de plantas transmitidos por Brevipalpus (Acari: Tenuipalpidae) - VTB: levantamento, identificação, caracterização molecular, filogenia; relações vírus/vetor/hospedeira; biologia, taxonomia e manejo do vetor
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Temático