| Full text | |
| Author(s): |
Ramos-Gonzalez, Pedro Luis
[1]
;
Sarubbi-Orue, Humberto
[2]
;
Gonzales-Segnana, Luis
[2]
;
Chabi-Jesus, Camila
[3, 1]
;
Freitas-Astua, Juliana
[4, 1]
;
Kitajima, Eliot Watanabe
[3]
Total Authors: 6
|
| Affiliation: | [1] IAC, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP - Brazil
[2] Univ Nacl Asuncion, Asuncion 1618 - Paraguay
[3] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Piracicaba, SP - Brazil
[4] Embrapa Cassava & Fruits, Cruz Das Almas, BA - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHYTOPATHOLOGY; v. 164, n. 5, p. 342-347, MAY 2016. |
| Web of Science Citations: | 7 |
| Abstract | |
The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. (AU) | |
| FAPESP's process: | 08/57909-2 - Genomic platforms applied to citrus breeding |
| Grantee: | Marcos Antonio Machado |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite |
| Grantee: | Elliot Watanabe Kitajima |
| Support Opportunities: | Research Projects - Thematic Grants |