Orchid Fleck Virus Infecting Orchids in Paraguay: First Report and Use of Degenerate Primers for its Detection

Author(s)

Ramos-Gonzalez, Pedro Luis ^[1] ; Sarubbi-Orue, Humberto ^[2] ; Gonzales-Segnana, Luis ^[2] ; Chabi-Jesus, Camila ^{[3, 1]} ; Freitas-Astua, Juliana ^{[4, 1]} ; Kitajima, Eliot Watanabe ^[3]

Affiliation

^[1] IAC, Ctr Citricultura Sylvio Moreira, Cordeiropolis, SP - Brazil

^[2] Univ Nacl Asuncion, Asuncion 1618 - Paraguay

^[3] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Piracicaba, SP - Brazil

^[4] Embrapa Cassava & Fruits, Cruz Das Almas, BA - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHYTOPATHOLOGY; v. 164, n. 5, p. 342-347, MAY 2016.
• Web of Science Citations: 7

Abstract

The presence of Orchid fleck virus (OFV) in Paraguay was confirmed in orchid plants collected during a survey carried out in 2013. Leaves displayed ringspot and fleck symptoms, and in infected tissues, non-enveloped, short, rodlike viral particles were observed. Partial OFV N and L genes were amplified using specific and degenerate primers, respectively; the nucleotide sequences demonstrated high identities (98%) with other OFV isolates. Degenerate primers for the L gene were designed considering conserved regions within all of the available OFV sequences and those from the closely related isolates citrus leprosis virus nuclear type (CiLV-N) and citrus necrotic spot virus. Degenerate primers were also successfully used for the detection of CiLV-N from infected citrus samples. (AU)

• FAPESP's process: 08/57909-2 - Genomic platforms applied to citrus breeding
• Grantee: Marcos Antonio Machado
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants