Characterization of plant viruses transmitted by the false spider mite Brevipalpus spp. (Tenuipalpidae) and studies on the virus/vector/plant relationship

• Grant number: 00/11805-0
• Support Opportunities: Research Projects - Thematic Grants
• Duration: July 01, 2001 - June 30, 2007
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Elliot Watanabe Kitajima
• Grantee: Elliot Watanabe Kitajima
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Beatriz Appezzato da Glória

Abstract

This proposal deals with several plant viruses transmitted by the tenuipalpidae mite Brevipalpus spp. and intends to give a significative advance on the knowledge of the virus/vector/host plant relationships including biological, pathological, biochemical, molecular, immunological, morphological, cytopathological aspects as well as resolving problems in taxonomy, epidemiology and control of the disease/vector. The most important member of this group is the citrus leprosis virus (CiLV), endemic in Brazil (and present in Argentina, Paraguay, Venezuela and more recently in Panama) and causing up to US$ 100 million/year to the growers in direct losses and mite control measurements. The previous work developed by the proposing group discovered 9 new diseases transmitted by the Brevipalpus mite in Brazil (in plants as passion flower, ivy, Hibiscus spp., Malvaviscum, Clerodendron, Schefflera, Solanum violaefolium adding to the 5 previously known (CiLV, Ligustrum ringspot, zonate chlorosis, orchid fleck and coffee ringspot) thus creating a possibility to study most of them together for comparison. The group has large experience with these viruses and the mite vector and has suitable facilities to carry out the research. Because there are still some technical difficulties in purifying and handling CiLV, the expectation is to find another easier to deal, to serve as model and through it better understand this group of virus. The final goal is to get epidemiological parameters which may lead to a more efficient and less expensive controle measure of diseases economically important as citrus leprosis and passion fruit green spot. It is a multidisciplinar, 4 year project involving areas as plant virology, acarology, molecular biology, plant anatomy/ultrastructure and epidemiology to be conducted by a team of experts coordinated by the proponent. Graduate and undergraduate students will also participate assuring the formation of more competence in plant virology, acarology, call biology, etc. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KITAJIMA, ELLIOT W.; GROOT, THOMAS V. M.; NOVELLI, VALDENICE M.; FREITAS-ASTÚA, JULIANA; ALBERTI, GERD; MORAES, GILBERTO J. DE. In situ observation of the Cardinium symbionts of Brevipalpus (Acari : Tenuipalpidae) by electron microscopy. Experimental and Applied Acarology, v. 42, n. 4, p. 263-271, Aug. 2007. (00/11805-0)

ELLIOT WATANABE KITAJIMA; KAREN SUMIRE KUBO; PAULO DE TARSO OLIVEIRA FERREIRA; BERENICE KUSSUMOTO DE ALCÂNTARA; ALESSANDRA JESUS BOARI; RENATA TAKASSUGI GOMES; JULIANA FREITAS-ASTUA; JORGE ALBERTO MARQUES REZENDE; GILBERTO JOSÉ DE MORAIS; RENATO BARBOSA SALAROLI. Mancha clorótica do Clerodendrum, uma enfermidade causada por um vírus do tipo nuclear, transmitido pelo ácaro Brevipalpus phoenicis (Acari:Tenuipalpidae). Scientia Agricola, v. 65, n. 1, p. 36-49, 2008-02-00. (00/11805-0)

KUBO, KAREN SUMIRE; STUART, R. M.; FREITAS-ASTUA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W.. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid gene. ARCHIVES OF VIROLOGY, v. 154, n. 6, p. 1009-1014, JUN 2009. (00/11805-0)

MARQUES, JOAO P. R.; KITAJIMA, ELLIOT W.; FREITAS-ASTUA, JULIANA; APPEZZATO-DA-GLORIA, BEATRIZ. Comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange. Anais da Academia Brasileira de Ciências, v. 82, n. 2, p. 501-511, JUN 2010. (00/11805-0)

RODRIGUES, JOSE CARLOS V.; ANTONY, LUCILLE M. K.; SALAROLI, RENATO B.; KITAJIMA, ELLIOT W.. Brevipalpus-associated viruses in the central Amazon Basin. TROPICAL PLANT PATHOLOGY, v. 33, n. 1, p. 12-19, JAN-FEB 2008. (00/11805-0)

RENATA TAKASSUGUI GOMES; ELLIOT WATANABE KITAJIMA; FRANCISCO ANDRÉ OSAMU TANAKA; JOÃO PAULO RODRIGUES MARQUES; BEATRIZ APPEZZATO-DA-GLÓRIA. Anatomia de lesões foliares causadas pelo vírus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies. Summa Phytopathologica, v. 36, n. 4, p. 291-297, 2010-12-00. (08/52691-9, 00/11805-0)