Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production

Autor(es)

Silveira, Erick A. ^{[1, 2]} ; Tardioli, Paulo W. ^[3] ; Farinas, Cristiane S. ^{[3, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Grad Program Biotechnol, BR-13565905 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 179, n. 4, p. 558-571, JUN 2016.
• Citações Web of Science: 4

Resumo

The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 A degrees C. This lipase presented good thermal stability up to 60 A degrees C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production. (AU)

• Processo FAPESP: 13/20826-0 - Produção de lipase pelo processo de fermentação combinada e sua aplicação na modificação de óleos e gorduras
• Beneficiário: Erick de Abreu Silveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado