Determination of pesticides in sugarcane juice employing microextraction by packed sorbent followed by gas chromatography and mass spectrometry

Autor(es)

Fumes, Bruno Henrique ; Andrade, Felipe Nascimento ; dos Santos Neto, Alvaro Jose ; Lancas, Fernando Mauro

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 39, n. 14, p. 2823-2830, JUL 2016.
• Citações Web of Science: 10

Resumo

This paper describes the development of a method for the determination of six pesticides (tebuthiuron, carbofuran, atrazine, metribuzine, ametryn, and bifenthrin) in sugarcane juice using microextraction by packed sorbent as the extraction technique. The extraction steps were optimized by factorial design, being the variables pH, ionic strength, desorption solvent and solvent volume optimized for comparisons among sorbent materials. Among the evaluated materials C18-Chromabond (R) showed better extraction efficiency. A factorial design 23 with central point was used for the extraction cycles optimization Draw/eject and washes cycles showed significant improvements in the extraction efficiency when the number of cycles increased. The method was validated and showed a limit of quantification in the range of 2.0-10.0 mu g.L-1. The calibration curves were constructed by weighting models that reduced the sum of absolute residues values and improved determination coefficient. The matrix factor and extraction efficiency were 97.3-77.3% and 27.1-64.8%, respectively. The accuracy was 71.7-106.9%; precision evaluated as the coefficient of variance obtained in intra and inter day analysis was 4.5-15.9%. The method was applied to the determination of pesticide residues in four sugarcane juice samples commercially available in markets from different cities from Sao Paulo state, Brazil. (AU)

• Processo FAPESP: 11/09898-4 - Desenvolvimento de polímeros molecularmente impressos e seu uso para o preparo de amostras e análise de triazinas e sulfoniluréias em milho utilizando cromatografia líquida de alta eficiência (HPLC).
• Beneficiário: Felipe Nascimento Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/19910-9 - Desenvolvimento de estratégias de química verde em cromatografia e aplicação na avaliação da degradação de antibióticos em águas residuárias por biorreatores anaeróbios de leito fixo
• Beneficiário: Alvaro José dos Santos Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/17425-4 - Avaliação do emprego da técnica MEPS na análise de agrotóxicos em caldo de cana-de-açúcar por GC-MS
• Beneficiário: Bruno Henrique Fumes
• Modalidade de apoio: Bolsas no Brasil - Mestrado