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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Chemical composition and antifungal potential of Brazilian propolis against Candida spp.

Texto completo
Autor(es):
Freires, I. A. ; Queiroz, V. C. P. P. ; Furletti, V. F. ; Ikegaki, M. ; de Alencar, S. M. ; Duarte, M. C. T. ; Rosalen, P. L.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL DE MYCOLOGIE MEDICALE; v. 26, n. 2, p. 122-132, JUN 2016.
Citações Web of Science: 16
Resumo

Propolis is known to have biological properties against numerous microorganisms of clinical interest. This study aimed to determine the chemical composition and antifungal activity of Brazilian propolis (types 3 and 13) against Candida spp. and their effects on the morphology of preformed and mature Candida biofilms. Samples of propolis (3 and 13) collected by Apis mellifera honeybees were obtained from different regions in Brazil. Ethanolic extracts of propolis (EEP) were prepared, fractionated and submitted to chemical analysis by GC/MS. The extracts and their hexane, dichloromethane and ethyl acetate fractions were tested for their ability to inhibit Candida spp. (C. albicans, C. dubliniensis, C. glabrata, C. kruzei, C. tropicalis and C. parapsilosis) by determination of the minimum inhibitory and fungicidal concentrations (MIC/MFC). Additionally, their effects on morphology of preformed and mature biofilms were observed by scanning electron microscopy. The phenolic compounds p-coumaric acid, caffeic acid phenethyl ester (CAPE), kaempferol and quercetin were identified in the EEP-3 and its bioactive dichloromethane fraction; and isoflavonoids such as medicarpin, vestitol and formononetin were found in the EEP-13, and triterpenes in its bioactive hexane fraction. The EEP-3 and EEP-13 and their bioactive fractions showed MIC values ranging from 0.2 to 125 mu g/mL and MFC values between 125 and 500 mu g/mL. The EEP and fractions were predominantly fungistatic agents. All extracts and fractions disrupted biofilm structures at 500 mu/mL and amorphous areas with cell damage were clearly observed in preformed and mature biofilms. Propolis types 3 and 13 have strong anti-Candida activity and should be considered as promising candidates to treat oral and systemic candidiasis. (C) 2016 Elsevier Masson SAS. All rights reserved. (AU)

Processo FAPESP: 04/08635-6 - Isolamento e identificação de compostos bioativos de uma nova variedade de própolis brasileira produzida por abelhas da espécie Apis mellifera
Beneficiário:Severino Matias de Alencar
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 13/25080-7 - Prevalência de cepas de Streptococcus mutans expressando Cnm de crianças e adultos e o papel desta glicoproteína nas interações patógeno-hospedeiro
Beneficiário:Irlan de Almeida Freires
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/51048-5 - Avaliação do potencial antifúngico de própolis de Apis mellifera contra leveduras do gênero Candida
Beneficiário:Marta Cristina Teixeira Duarte
Modalidade de apoio: Auxílio à Pesquisa - Regular