PRELIMINARY REPORT ON THE PUTATIVE ASSOCIATION OF IL10-3575 T/A GENETIC POLYMORPHISM WITH MALARIA SYMPTOMS

Autor(es)

Domingues, Wilson ; Kanunfre, Kelly Aparecida ; Rodrigues, Jonatas Cristian ; Teixeira, Leandro Emidio ; Yamamoto, Lidia ; Okay, Thelma Suely

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 58, 2016.
• Citações Web of Science: 2

Resumo

Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play a key role. This study has determined the frequency of single nucleotide polymorphisms (SNPs) in some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) and TGF beta 1 (+869; rs1800470), by means of PCR-RFLP. Blood samples were collected from 104 symptomatic and 37 asymptomatic subjects. Laboratory diagnosis was assessed by the thick blood smear test and nested-PCR. No association was found between IL6 (-174), IL12p40 (+1188), IL4 (+33), IL10 (-3575), TGF beta 1 (+869) SNPs and malaria symptoms. However, regarding the IL10 -3575 T/A SNP, there were significantly more AA and AT subjects, carrying the polymorphic allele A, in the symptomatic group (chi(2) = 4.54, p = 0.01, OR = 0.40 {[}95% CI - 0.17- 0.94]). When the analysis was performed by allele, the frequency of the polymorphic allele A was also significantly higher in the symptomatic group (chi(2) = 4.50, p = 0.01, OR = 0.45 {[}95% CI - 0.21-0.95]). In conclusion, this study has suggested the possibility that the IL10 - 3575 T/A SNP might be associated with the presence and maintenance of malaria symptoms in individuals living in endemic areas. Taking into account that this polymorphism is related to decreased IL10 production, a possible role of this SNP in the pathophysiology of malaria is also suggested, but replication studies with a higher number of patients and evaluation of IL10 levels are needed for confirmation. (AU)

• Processo FAPESP: 10/15022-1 - Frequência de alelos do HLA-DQA1 e DQB1 em fetos com toxoplasmose: associação com os genótipos parasitários e a carga parasitária
• Beneficiário: Thelma Suely Okay
• Modalidade de apoio: Auxílio à Pesquisa - Regular