| Full text | |
| Author(s): |
Domingues, Wilson
;
Kanunfre, Kelly Aparecida
;
Rodrigues, Jonatas Cristian
;
Teixeira, Leandro Emidio
;
Yamamoto, Lidia
;
Okay, Thelma Suely
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 58, 2016. |
| Web of Science Citations: | 2 |
| Abstract | |
Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play a key role. This study has determined the frequency of single nucleotide polymorphisms (SNPs) in some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) and TGF beta 1 (+869; rs1800470), by means of PCR-RFLP. Blood samples were collected from 104 symptomatic and 37 asymptomatic subjects. Laboratory diagnosis was assessed by the thick blood smear test and nested-PCR. No association was found between IL6 (-174), IL12p40 (+1188), IL4 (+33), IL10 (-3575), TGF beta 1 (+869) SNPs and malaria symptoms. However, regarding the IL10 -3575 T/A SNP, there were significantly more AA and AT subjects, carrying the polymorphic allele A, in the symptomatic group (chi(2) = 4.54, p = 0.01, OR = 0.40 {[}95% CI - 0.17- 0.94]). When the analysis was performed by allele, the frequency of the polymorphic allele A was also significantly higher in the symptomatic group (chi(2) = 4.50, p = 0.01, OR = 0.45 {[}95% CI - 0.21-0.95]). In conclusion, this study has suggested the possibility that the IL10 - 3575 T/A SNP might be associated with the presence and maintenance of malaria symptoms in individuals living in endemic areas. Taking into account that this polymorphism is related to decreased IL10 production, a possible role of this SNP in the pathophysiology of malaria is also suggested, but replication studies with a higher number of patients and evaluation of IL10 levels are needed for confirmation. (AU) | |
| FAPESP's process: | 10/15022-1 - Frequency of HLA-DQA1 and DQB1 alleles in fetuses with toxoplasmosis: association with the parasite genotype and the parasite load |
| Grantee: | Thelma Suely Okay |
| Support Opportunities: | Regular Research Grants |