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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PRELIMINARY REPORT ON THE PUTATIVE ASSOCIATION OF IL10-3575 T/A GENETIC POLYMORPHISM WITH MALARIA SYMPTOMS

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Author(s):
Domingues, Wilson ; Kanunfre, Kelly Aparecida ; Rodrigues, Jonatas Cristian ; Teixeira, Leandro Emidio ; Yamamoto, Lidia ; Okay, Thelma Suely
Total Authors: 6
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 58, 2016.
Web of Science Citations: 2
Abstract

Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play a key role. This study has determined the frequency of single nucleotide polymorphisms (SNPs) in some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) and TGF beta 1 (+869; rs1800470), by means of PCR-RFLP. Blood samples were collected from 104 symptomatic and 37 asymptomatic subjects. Laboratory diagnosis was assessed by the thick blood smear test and nested-PCR. No association was found between IL6 (-174), IL12p40 (+1188), IL4 (+33), IL10 (-3575), TGF beta 1 (+869) SNPs and malaria symptoms. However, regarding the IL10 -3575 T/A SNP, there were significantly more AA and AT subjects, carrying the polymorphic allele A, in the symptomatic group (chi(2) = 4.54, p = 0.01, OR = 0.40 {[}95% CI - 0.17- 0.94]). When the analysis was performed by allele, the frequency of the polymorphic allele A was also significantly higher in the symptomatic group (chi(2) = 4.50, p = 0.01, OR = 0.45 {[}95% CI - 0.21-0.95]). In conclusion, this study has suggested the possibility that the IL10 - 3575 T/A SNP might be associated with the presence and maintenance of malaria symptoms in individuals living in endemic areas. Taking into account that this polymorphism is related to decreased IL10 production, a possible role of this SNP in the pathophysiology of malaria is also suggested, but replication studies with a higher number of patients and evaluation of IL10 levels are needed for confirmation. (AU)

FAPESP's process: 10/15022-1 - Frequency of HLA-DQA1 and DQB1 alleles in fetuses with toxoplasmosis: association with the parasite genotype and the parasite load
Grantee:Thelma Suely Okay
Support type: Regular Research Grants