Anti-inflammatory activity and polyphenolic profile of the hydroalcoholic seed extract of Eugenia leitonii, an unexplored Brazilian native fruit

Autor(es)

Lazarini, Josy Goldoni ; Franchin, Marcelo ; Infante, Juliana ; Rizzato Paschoal, Jonas Augusto ; Freires, Irlan Almeida ; de Alencar, Severino Matias ; Rosalen, Pedro Luiz

• Tipo de documento: Artigo Científico
• Fonte: Journal of Functional Foods; v. 26, p. 249-257, OCT 2016.
• Citações Web of Science: 5

Resumo

This study determined the anti-inflammatory activity, mechanisms of action, and polyphenolic profile of Eugenia leitonii seed extract. The total phenolic content of the hydroethanolic extract from E. leitonii seeds (ESEL) was measured, and its phenolic compounds were identified by LC-MS/MS. The ESEL was submitted to in vitro (RAW264.7) and in vivo assays on neutrophil migration; expression of P-selectin and ICAM-1; release of TNF-alpha, IL-1 beta, and CXCL2/ MIP-2; NF-kappa B activation; and carrageenan-induced paw oedema. ESEL yielded total phenolic content of 158.74 +/- 3.5 mg GAE/g, with the presence of flavonols, anthocyanins and ellagitannins. ESEL significantly reduced neutrophil migration; decreased the expression of P-selectin and ICAM-1; decreased the release of TNF-alpha and CXCL2/MIP-2, as well as NF-kappa B activation and paw oedema in mice. Thus, this extract showed to be a promising source of bioactive compounds with anti-inflammatory potential by inhibiting neutrophil migration through a decrease in the release of TNF-alpha and CXCL2/MIP-2 (NF-kappa B pathway) in macrophages. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/26251-0 - Prospecção de atividade anti-inflamatória em espécies frutíferas nativas do Brasil
• Beneficiário: Josy Goldoni Lazarini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/13190-2 - Composição fenólica e potencial biológico, in vitro e in vivo, de frutíferas nativas do Brasil
• Beneficiário: Severino Matias de Alencar
• Modalidade de apoio: Auxílio à Pesquisa - Regular