Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production

Autor(es)

Goldbeck, Rosana ; Goncalves, Thiago A. ; Damasio, Andre R. L. ; Brenelli, Livia B. ; Wolf, Lucia D. ; Paixao, Douglas A. A. ; Rocha, George J. M. ; Squina, Fabio M.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC; v. 131, p. 36-46, SEP 2016.
• Citações Web of Science: 6

Resumo

Enzymatic hydrolysis of lignocellulosic biomass is limited by economic considerations arising from enzyme production costs and specific activities. The effect of six hemicellulases on raw sugarcane bagasse and two types of pretreated sugarcane bagasse was investigated using experimental designs. Our strategy was successful for developing more efficient and less expensive enzymatic mixture, and also revealed that hemicellulase mixtures with multiple activities could be less effective than expected. In this study, only two hemicelulases, the endo-1,4-xylanases (GH11) from Penicillium funiculosum (XynC11/CAC15487) and the feruloyl esterase (CE1) from Clostridium thermocellum (CtFAEIATCC27405), effectively broke down hemicellulose from pretreated sugarcane bagasse (up to 65%), along with the production of xylooligosaccharides (XOS). Our results also demonstrated that GH11 and CE1 can improve biomass saccharification by cellulases. Treatment with these two enzymes followed by a commercial cellulase cocktail (Accellerase (R) 1500) increased saccharifi cation of pretreated lignocellulose by 24%. Collectively, our data contributes to the rational design of more efficient and less expensive enzyme mixtures, targeting the viable production of bioethanol and other biorefinery products. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/20549-4 - Secreção de glicoproteínas heterólogas em Aspergillus: efeito do padrão de glicosilação em parâmetros funcionais de glicosil hidrolases
• Beneficiário: André Ricardo de Lima Damasio
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
• Processo FAPESP: 12/18859-5 - Desenvolvimento de um modelo enzimático para desconstrução da parede celular de bagaço de cana de açúcar
• Beneficiário: Rosana Goldbeck
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado