Effects of the pectinolytic activity of pectin methyl esterase-CBM3 chimeras on th...
| Full text | |
| Author(s): |
Goldbeck, Rosana
;
Goncalves, Thiago A.
;
Damasio, Andre R. L.
;
Brenelli, Livia B.
;
Wolf, Lucia D.
;
Paixao, Douglas A. A.
;
Rocha, George J. M.
;
Squina, Fabio M.
Total Authors: 8
|
| Document type: | Journal article |
| Source: | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC; v. 131, p. 36-46, SEP 2016. |
| Web of Science Citations: | 6 |
| Abstract | |
Enzymatic hydrolysis of lignocellulosic biomass is limited by economic considerations arising from enzyme production costs and specific activities. The effect of six hemicellulases on raw sugarcane bagasse and two types of pretreated sugarcane bagasse was investigated using experimental designs. Our strategy was successful for developing more efficient and less expensive enzymatic mixture, and also revealed that hemicellulase mixtures with multiple activities could be less effective than expected. In this study, only two hemicelulases, the endo-1,4-xylanases (GH11) from Penicillium funiculosum (XynC11/CAC15487) and the feruloyl esterase (CE1) from Clostridium thermocellum (CtFAEIATCC27405), effectively broke down hemicellulose from pretreated sugarcane bagasse (up to 65%), along with the production of xylooligosaccharides (XOS). Our results also demonstrated that GH11 and CE1 can improve biomass saccharification by cellulases. Treatment with these two enzymes followed by a commercial cellulase cocktail (Accellerase (R) 1500) increased saccharifi cation of pretreated lignocellulose by 24%. Collectively, our data contributes to the rational design of more efficient and less expensive enzyme mixtures, targeting the viable production of bioethanol and other biorefinery products. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases |
| Grantee: | André Ricardo de Lima Damasio |
| Support type: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 12/18859-5 - Development of enzymatic model for deconstruction of sugarcane bagasse cell wall |
| Grantee: | Rosana Goldbeck |
| Support type: | Scholarships in Brazil - Post-Doctorate |