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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Proteomic and Glycoproteomic Profilings Reveal That Post- translational Modifications of Toxins Contribute to Venom Phenotype in Snakes

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Autor(es):
Andrade-Silva, Debora ; Zelanis, Andre ; Kitano, Eduardo S. ; Junqueira-de-Azevedo, Inacio L. M. ; Reis, Marcelo S. ; Lopes, Aline S. ; Serrano, Solange M. T.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PROTEOME RESEARCH; v. 15, n. 8, p. 2658-2675, AUG 2016.
Citações Web of Science: 10
Resumo

Snake venoms are biological weapon systems composed of secreted proteins and peptides that are used for immobilizing or killing prey. Although post-translational modifications are widely investigated because of their importance in many biological phenomena, we currently still have little understanding of how protein glycosylation impacts the variation and stability of venom proteomes. To address these issues, here we characterized the venom proteomes of seven Bothrops snakes using a shotgun proteomics strategy. Moreover, we compared the electrophoretic profiles of native and deglycosylated venoms and, in order to assess their subproteomes of glycoproteins, we identified the proteins with affinity for three lectins with different saccharide specificities and their putative glycosylation sites. As proteinases are abundant glycosylated toxins, we examined the effect of N-deglycosylation on their catalytic activities and show that the proteinases of the seven venoms were similarly affected by removal of N-glycans. Moreover, we prospected putative glycosylation sites of transcripts of a B. jararaca venom gland data set and detected toxin family related patterns of glycosylation. Based on our global analysis, we report that Bothrops venom proteomes and glycoproteomes contain a core of components that markedly define their composition, which is conserved upon evolution in parallel to other molecular markers that determine their phylogenetic classification. (AU)

Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 13/13548-4 - Caracterização proteômica / glicoproteômica de venenos de serpentes do complexo Bothrops jararaca com ênfase no N-terminoma e N-glicoma de toxinas
Beneficiário:Solange Maria de Toledo Serrano
Linha de fomento: Auxílio à Pesquisa - Regular