Photobiomodulation therapy on collagen type I and III, vascular endothelial growth factor, and metalloproteinase in experimentally induced tendinopathy in aged rats

Autor(es)

de Farias Marques, Anna Cristina ; Albertini, Regiane ; Serra, Andrey Jorge ; Pereira da Silva, Evela Aparecida ; Cavalcante de Oliveira, Vanessa Lima ; Silva, Luciana Miatto ; Pinto Leal-Junior, Ernesto Cesar ; Camillo de Carvalho, Paulo de Tarso

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 9, p. 1915-1923, DEC 2016.
• Citações Web of Science: 3

Resumo

This study investigates the effect of photobiomodulation therapy (PBMT) on collagen type I and III, matrix metalloproteinase (MMP), and vascular endothelial growth factor (VEGF) in experimentally induced tendinopathy in female aged rats. Tendinopathy was induced by the Achilles tendoncollagenase peritendinous. Forty-two Wistar rats (Norvegicus albinus) were used; groups consisted of 36 aged animals (18 months old; mean body weight, 517.7 +/- 27.54 g) and 6 adult animals (12 weeks old; mean body weight, 266 +/- 19.30 g). The animals were divided into three groups: control, aged tendinopathy, and aged tendinopathy PBMT; the aged groups were subdivided based on time to euthanasia: 7, 14, and 21 days. PBMT involved a gallium-arsenide-aluminum laser (Theralaser, DMCA (R)) with active medium operating at wavelength 830 A +/- 10 nm, 50 mW power, 0.028 cm(2) laser beam, 107 J/cm(2) energy density, 1.8 W/cm(2) power density, and an energy of 3 J per point. The laser was applied by direct contact with the left Achilles tendon during 60 s per point at a frequency of three times per week, until the euthanasia date (7, 14, and 21 days). VEGF, MMP-3, and MMP-9 were analyzed by immunohistochemistry, and collagen type I and III by Sirius red. PBMT increased the deposition of collagen type I and III in a gradual manner, with significant differences relative to the group aged tendonitis (p < 0.001), and in relation to VEGF (p < 0.001); decreased expression of MMP-3 and 9 were observed in group aged tendinopathy (p < 0.001). PBMT, therefore, increased the production of collagen type I and III, downregulated the expression of MMP-3 and MMP-9, and upregulated that of VEGF, with age and age-induced hormonal deficiency. (AU)

• Processo FAPESP: 15/13677-4 - Efeitos da Laserterapia de Baixa Intensidade na Biodisponibilidade de Células-Tronco Mesenquimais Derivadas de Adipócitos e Implantadas em Joelhos de Ratos Submetidos a Osteoartrite Experimental
• Beneficiário: Paulo de Tarso Camillo de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular