Variation in myogenic differentiation 1 mRNA abundance isassociated with beef tenderness in Nelore cattle

Autor(es)

Tizioto, P. C. ; Coutinho, L. L. ; Mourao, G. B. ; Gasparin, G. ; Malago-, Jr., W. ; Bressani, F. A. ; Tullio, R. R. ; Nassu, R. T. ; Taylor, J. F. ; Regitano, L. C. A.

• Tipo de documento: Artigo Científico
• Fonte: ANIMAL GENETICS; v. 47, n. 4, p. 491-494, AUG 2016.
• Citações Web of Science: 0

Resumo

The myogenic differentiation 1 gene (MYOD1) has a key role in skeletal muscle differentiation and composition through its regulation of the expression of several muscle-specific genes. We first used a general linear mixed model approach to evaluate the association of MYOD1 expression levels on individual beef tenderness phenotypes. MYOD1mRNA levels measured by quantitative polymerase chain reactions in 136 Nelore steers were significantly associated (P0.01) with Warner-Bratzler shear force, measured on the longissimus dorsi muscle after 7 and 14days of beef aging. Transcript abundance for the muscle regulatory gene MYOD1 was lower in animals with more tender beef. We also performed a co-expression network analysis using whole transcriptome sequence data generated from 30 samples of longissimus muscle tissue to identify genes that are potentially regulated by MYOD1. The effect of MYOD1 gene expression on beef tenderness may emerge from its function as an activator of muscle-specific gene transcription such as for the serum response factor (C-fos serum response element-binding transcription factor) gene (SRF), which determines muscle tissue development, composition, growth and maturation. (AU)

• Processo FAPESP: 10/06515-4 - Prospecção e validação de SNPs em genes candidatos para maciez de carne em famílias de referência da raça Nelore
• Beneficiário: Polyana Cristine Tizioto
• Modalidade de apoio: Bolsas no Brasil - Doutorado