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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MicroRNAs-424 and 206 are potential prognostic markers in spinal onset amyotrophic lateral sclerosis

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Autor(es):
de Andrade, Helen M. T. ; de Albuquerque, Milena ; Avansini, Simoni H. ; Rocha, Cristiane de S. ; Dogini, Danyella B. ; Nucci, Anamarli ; Carvalho, Benilton ; Lopes-Cendes, Iscia ; Franca, Jr., Marcondes C.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF THE NEUROLOGICAL SCIENCES; v. 368, p. 19-24, SEP 15 2016.
Citações Web of Science: 19
Resumo

Introduction: Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation. Objectives: To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression. Methods: Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data. Results: We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time. Conclusion: MiR-424 and miR-206 are potential prognostic markers for ALS. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/01766-7 - Contribuição ao diagnóstico, à fisiopatologia e à terapêutica das neuronopatias sensitivas
Beneficiário:Marcondes Cavalcante Franca Junior
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores