| Full text | |
| Author(s): |
de Andrade, Helen M. T.
;
de Albuquerque, Milena
;
Avansini, Simoni H.
;
Rocha, Cristiane de S.
;
Dogini, Danyella B.
;
Nucci, Anamarli
;
Carvalho, Benilton
;
Lopes-Cendes, Iscia
;
Franca, Jr., Marcondes C.
Total Authors: 9
|
| Document type: | Journal article |
| Source: | JOURNAL OF THE NEUROLOGICAL SCIENCES; v. 368, p. 19-24, SEP 15 2016. |
| Web of Science Citations: | 19 |
| Abstract | |
Introduction: Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation. Objectives: To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression. Methods: Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data. Results: We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time. Conclusion: MiR-424 and miR-206 are potential prognostic markers for ALS. (C) 2016 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 13/01766-7 - Sensory neuronopathies: investigation of new diagnostic methods, mechanisms of the disease and therapeutic strategies |
| Grantee: | Marcondes Cavalcante Franca Junior |
| Support Opportunities: | Research Grants - Young Investigators Grants |