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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Physico-chemical stability and in vitro digestibility of beta-carotene-loaded lipid nanoparticles of cupuacu butter (Theobroma grandiflorum) produced by the phase inversion temperature (PIT) method

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Autor(es):
Gomes, Graziela V. L. ; Sola, Mirella R. ; Marostegan, Luis F. P. ; Jange, Camila G. ; Cazado, Camila P. S. ; Pinheiro, Ana C. ; Vicente, Antonio A. ; Pinho, Samantha C.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Journal of Food Engineering; v. 192, p. 93-102, JAN 2017.
Citações Web of Science: 10
Resumo

Beta-carotene is a carotenoid with a wide spectrum of biological activities (e.g., anti-cancer, anti-hypertensive, and anti-inflammatory). However, because of its extremely high hydrophobicity, it is difficult to incorporate in food formulations and its bioavailability is fairly low. Lipid-based encapsulation colloidal systems such as lipid nanoparticles can help overcome these issues. In this study, beta-carotene loaded lipid nanoparticles were produced by the phase inversion temperature (PIT) method from 10% cupuacu butter and 20% surfactant (Cremophor RH40 and Span 80). The inversion temperature of the nanoparticles was 74 degrees C and their average diameter was 35 nm. After 100 days of storage, 85% of the initial amount of beta-carotene remained in the nanoparticles; alpha-tocopherol was found to be essential for carotenoid preservation. Comparison of the results of in vitro digestion between static and dynamic systems was performed, and the characteristics of each digestion system led to diverse results in terms of average particle size and beta-carotene bioaccessibility. Although the static system was much simpler than the dynamic system, it could not provide reliable data of the digestibility of the lipid nanoparticles. The bioaccessibility of beta-carotene in the static system was 92%, very similar to the results found in the literature; by comparison, the dynamic system revealed a beta-carotene bioaccessibility of nearly 20%. Despite this discrepancy, the highly realistic conditions of digestion simulated by the dynamic in vitro system indicate that the results of this system are more reliable than those obtained from the simplified static system applied in this research. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/20523-0 - Nanopartículas lipídicas sólidas produzidas por método de baixa energia: otimização das condições de processo, avaliação da absorção de bioativo encapsulado e estudo de digestibilidade in vitro
Beneficiário:Graziela Veiga de Lara Gomes
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/01260-0 - Estudo de viabilidade da produção de Nanoparticulas Lipídicas Sólidas pelo método da temperatura de transição de fases (método PIT)
Beneficiário:Mirella Roberta Sola
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 12/18495-3 - Digestibilidade in vitro dinâmica de nanopartículas lipídicas sólidas encapsulando beta-caroteno
Beneficiário:Graziela Veiga de Lara Gomes
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado