Neuroprotective Effects of Pomegranate Peel Extract after Chronic Infusion with Amyloid-beta Peptide in Mice

Autor(es)

Morzelle, Maressa Caldeira ; Salgado, Jocelem Mastrodi ; Telles, Milena ; Mourelle, Danilo ; Bachiega, Patricia ; Buck, Hudson Sousa ; Viel, Tania Araujo

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 11 NOV 9 2016.
• Citações Web of Science: 17
• Assunto(s): Doença de Alzheimer Radicais livres Fator neurotrófico ciliar

Resumo

Alzheimer's disease is a chronic and degenerative condition that had no treatment until recently. The current therapeutic strategies reduce progression of the disease but are expensive and commonly cause side effects that are uncomfortable for treated patients. Functional foods to prevent and/or treat many conditions, including neurodegenerative diseases, represent a promising field of study currently gaining attention. To this end, here we demonstrate the effects of pomegranate (Punica granatum) peel extract (PPE) regarding spatial memory, biomarkers of neuroplasticity, oxidative stress and inflammation in a mouse model of neurodegeneration. Male C57Bl/6 mice were chronically infused for 35 days with amyloid-beta peptide 1-42 (A beta) or vehicle (control) using mini-osmotic pumps. Another group, also infused with A beta, was treated with PPE (p.o. -beta A+PPE, 800 mg/kg/day). Spatial memory was evaluated in the Barnes maze. Animals treated with PPE and in the control group exhibited a reduction in failure to find the escape box, a finding that was not observed in the A beta group. The consumption of PPE reduced amyloid plaque density, increased the expression of neurotrophin BDNF and reduced the activity of acetylcholinesterase enzyme. A reduction in lipid peroxidation and in the concentration of the pro-inflammatory cytokine TNF-alpha was also observed in the PPE group. No hepatic lesions were observed in animals treated with PPE. In conclusion, administration of pomegranate peel extract has neuroprotective effects involving multiple mechanisms to prevent establishment and progression of the neurodegenerative process induced by infusion with amyloid-beta peptide in mice. (AU)

• Processo FAPESP: 12/12917-3 - Estudo das alterações moleculares colinérgicas que ocorrem após a aplicação de estratégias não-farmacológicas para melhora da memória
• Beneficiário: Tânia Araújo Viel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08989-1 - Efeito neuroprotetor da casca de romã (Punica granatum)
• Beneficiário: Maressa Caldeira Morzelle
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/13656-1 - Estudo do possível papel neuroprotetor do receptor B2 para bradicinina na Doença de Alzheimer
• Beneficiário: Hudson de Sousa Buck
• Modalidade de apoio: Auxílio à Pesquisa - Regular