Neovestitol, an isoflavonoid isolated from Brazilian red propolis, reduces acute and chronic inflammation: involvement of nitric oxide and IL-6

Autor(es)

Franchin, Marcelo ; Colon, David F. ; da Cunha, Marcos G. ; Castanheira, Fernanda V. S. ; Saraiva, Andre L. L. ; Bueno-Silva, Bruno ; Alencar, Severino M. ; Cunha, Thiago M. ; Rosalen, Pedro L.

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, NOV 7 2016.
• Citações Web of Science: 9

Resumo

Isoflavonoids have been largely studied due to their distinct biological activities identified thus far. Herein, we evaluated the activity of neovestitol, an isoflavonoid isolated from Brazilian red propolis, in acute and chronic inflammation. As for acute inflammation, we found that neovestitol reduced neutrophil migration, leukocyte rolling and adhesion, as well as expression of ICAM-1 in the mesenteric microcirculation during lipopolysaccharide-induced acute peritonitis. No changes were observed in the levels of TNF-alpha, CXCL1/KC and CXCL2/MIP-2 upon pretreatment with neovestitol. The administration of an inducible nitric oxide synthase (iNOS) inhibitor abolished the inhibitory effects of neovestitol in neutrophil migration and ICAM-1 expression. Nitrite levels increased upon treatment with neovestitol. No effects of neovestitol were observed on the chemotaxis of neutrophils in vitro. As for chronic inflammation, neovestitol also reduced the clinical score and joint damage in a collagen-induced arthritis model. There was no change in the frequency of IL-17-producing TCD4+ cells. In addition, pretreatment with neovestitol reduced the levels of IL-6. These results demonstrate a potential anti-inflammatory activity of neovestitol, which may be useful for therapeutic purposes and/or as a nutraceutical. (AU)

• Processo FAPESP: 12/22378-2 - Estudo in vivo e in vitro da atividade de compostos isolados de própolis brasileiras sobre a modulação da migração de neutrófilos no processo inflamatório
• Beneficiário: Pedro Luiz Rosalen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/01365-0 - Estudo in vivo e in vitro da atividade de compostos isolados de própolis brasileiras sobre a modulação da migração de neutrófilos no processo inflamatório
• Beneficiário: Marcelo Franchin
• Modalidade de apoio: Bolsas no Brasil - Doutorado