Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model

Autor(es)

Singulani, Junya de Lacorte ; Scorzoni, Liliana ; Alves de Paula e Silva, Ana Carolina ; Fusco-Almeida, Ana Marisa ; Soares Mendes-Giannini, Maria Jose

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS; v. 48, n. 3, p. 292-297, SEP 2016.
• Citações Web of Science: 11

Resumo

Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved. (AU)

• Processo FAPESP: 13/10917-9 - Modelos animais alternativos: Virulência de diferentes espécies filogenéticas de Paracoccidioides e efeito das proteínas 43kDa e 30kDa e de seus anticorpos
• Beneficiário: Liliana Scorzoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/10446-9 - Pesquisa de novas terapias e de biomarcadores na paracoccidioidomicose: estudo da atividade antifúngica de galatos de alquila em modelos alternativos e camundongos e identificação de microRNAs circulantes
• Beneficiário: Junya de Lacorte Singulani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/03700-9 - Animais alternativos aplicados à interação Paracoccidioides-hospedeiro para o desenvolvimento de estudos de virulência, eficácia e toxicidade de compostos antifúngicos e tratamentos preventivos
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular