Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding

Autor(es)

Medeiros, Ane H. ; Mingossi, Fabiana B. ; Dias, Renata O. ; Franco, Flavia P. ; Vicentini, Renato ; Mello, Marcia O. ; Moura, Daniel S. ; Silva-Filho, Marcio C.

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 17, n. 9 SEP 2016.
• Citações Web of Science: 3

Resumo

Sugarcane's (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory. (AU)

• Processo FAPESP: 98/15534-9 - Construção, seleção e caracterização de variantes do gene que codifica o inibidor de proteinase do tipo Bowman-Birk de soja contra a tripsina da Broca-da-cana (Diatraea saccharalis)
• Beneficiário: Marcia Ometto de Mello Alves José
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/15920-2 - Estudos funcionais de inibidores de proteases e serino proteases de plantas envolvidas na resposta a herbivoria
• Beneficiário: Fabiana Bombonato Mingossi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/12577-0 - Estudo das bases moleculares da ação da SUGARWIN em fungos fitopatogênicos
• Beneficiário: Flávia Pereira Franco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50275-9 - Decifrando os mecanismos moleculares envolvidos na localização subcelular de proteínas e na complexa interação planta-insetos-patógenos
• Beneficiário: Márcio de Castro Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 04/09713-0 - Estudo em larga escala do perfil de expressao genica da cana-de-acucar em resposta a diferentes estresses usando macroarranjos de dna.
• Beneficiário: Ane Hackbart de Medeiros
• Modalidade de apoio: Bolsas no Brasil - Doutorado