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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding

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Author(s):
Medeiros, Ane H. ; Mingossi, Fabiana B. ; Dias, Renata O. ; Franco, Flavia P. ; Vicentini, Renato ; Mello, Marcia O. ; Moura, Daniel S. ; Silva-Filho, Marcio C.
Total Authors: 8
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 17, n. 9 SEP 2016.
Web of Science Citations: 3
Abstract

Sugarcane's (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory. (AU)

FAPESP's process: 14/50275-9 - Deciphering the molecular mechanisms involved in the localization of organelar proteins as well as the complex plant-insect-pathogen interactions
Grantee:Márcio de Castro Silva Filho
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 13/12577-0 - Study of the molecular basis of SUGARWIN action in phytopathogenic fungi
Grantee:Flávia Pereira Franco
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/15920-2 - Functional studies of plant proteinase inhibitors and serine proteases involved in response to herbivore insects
Grantee:Fabiana Bombonato Mingossi
Support type: Scholarships in Brazil - Doctorate