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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gastrointestinal Transcriptomic Response of Metabolic Vitamin B12 Pathways in Roux-en-Y Gastric Bypass

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Sala, Priscila ; Belarmino, Giliane ; Torrinhas, Raquel S. ; Machado, Natasha M. ; Fonseca, Danielle C. ; Ravacci, Graziela R. ; Ishida, Robson K. ; Guarda, Ismael F. M. S. ; de Moura, Eduardo G. ; Sakai, Paulo ; Santo, Marco A. ; da Silva, Ismael D. C. G. ; Pereira, Claudia C. A. ; Logullo, Angela F. ; Heymsfield, Steven ; Giannella-Neto, Daniel ; Waitzberg, Dan L.
Número total de Autores: 17
Tipo de documento: Artigo Científico
Fonte: CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY; v. 8, JAN 2017.
Citações Web of Science: 5
Resumo

OBJECTIVES: Vitamin B12 (B12) deficiency after Roux-en-Y gastric bypass (RYGB) is highly prevalent and may contribute to postoperative complications. Decreased production of intrinsic factor owing to gastric fundus removal is thought to have a major role, but other components of B12 metabolism may also be affected. We evaluated changes in the expression levels of multiple B12 pathway-encoding genes in gastrointestinal (GI) tissues to evaluate the potential roles in contributing to post-RYGB B12 deficiency. METHODS: During double-balloon enteroscopy, serial GI biopsies were collected from 20 obese women (age, 46.9 +/- 6.2 years; body mass index, 46.5 +/- 5.3 kg/m(2)) with adult-onset type 2 diabetes (fasting plasma glucose >= 126 mg/dl; hemoglobin A1c >= 6.5%) before and, at the same site, 3 months after RYGB. Gene expression levels were assessed by the Affymetrix Human GeneChip 1.0 ST microarray. Findings were validated by real-time quantitative PCR (RT-qPCR). RESULTS: Gene expression levels with significant changes (P <= 0.05) included: transcobalamin I (TCN1) in remnant (-1.914-fold) and excluded (-1.985-fold) gastric regions; gastric intrinsic factor (GIF) in duodenum (-0.725-fold); and cubilin (CUBN) in duodenum (+0.982-fold), jejunum (+1.311-fold), and ileum (+0.685-fold). Validation by RT-qPCR confirmed (P <= 0.05) observed changes for TCN1 in the remnant gastric region (-0.132-fold) and CUBN in jejunum (+2.833-fold). CONCLUSIONS: RYGB affects multiple pathway-encoding genes that may be associated with postoperative B12 deficiency. Decreased TCN1 levels seem to be the main contributing factor. Increased CUBN levels suggest an adaptive genetic reprogramming of intestinal tissue aiming to compensate for impaired intestinal B12 delivery. (AU)

Processo FAPESP: 13/03246-0 - Estudo genômico, proteômico e metabolômico do tecido gastrointestinal de portadores de Diabetes melito tipo 2 após gastroplastia redutora a Y-Roux
Beneficiário:Priscila Sala Kobal
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 11/09612-3 - Alterações na expressão gênica do tecido gástrico e intestinal de portadores de Diabetes melito tipo 2 submetidos à gastroplastia redutora a Y-Roux
Beneficiário:Dan Linetzky Waitzberg
Linha de fomento: Auxílio à Pesquisa - Temático