Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis

Autor(es)

Avanzi, Ingrid Regina ; Gracioso, Louise Hase ; Galluzzi Baltazar, Marcela dos Passos ; Karolski, Bruno ; Perpetuo, Elen Aquino ; Oller do Nascimento, Claudio Augusto

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 24, n. 4, p. 3717-3726, FEB 2017.
• Citações Web of Science: 8

Resumo

Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level. Even though some results were ambiguous, accuracy can be improved by enhancing reference databases. Therefore, mass spectra analysis provides a reliable method to facilitate monitoring of the microbiota from copper-polluted sites. The understanding of the microbial community diversity in coppercontaminated sites can be helpful to understand the impact of the metal on the microbiome and to design bioremediation processes. (AU)

• Processo FAPESP: 12/06600-7 - Estudo da atividade microbiana na biorremediação de metais pesados na região amazônica
• Beneficiário: Ingrid Regina Avanzi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/11020-2 - Caracterização da comunidade bacteriana de uma área de processamento de cobre e identificação do mecanismo de resistência a metais pesados
• Beneficiário: Louise Hase Gracioso
• Modalidade de apoio: Bolsas no Brasil - Doutorado