Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis

Author(s)

Avanzi, Ingrid Regina ; Gracioso, Louise Hase ; Galluzzi Baltazar, Marcela dos Passos ; Karolski, Bruno ; Perpetuo, Elen Aquino ; Oller do Nascimento, Claudio Augusto

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 24, n. 4, p. 3717-3726, FEB 2017.
• Web of Science Citations: 8

Abstract

Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level. Even though some results were ambiguous, accuracy can be improved by enhancing reference databases. Therefore, mass spectra analysis provides a reliable method to facilitate monitoring of the microbiota from copper-polluted sites. The understanding of the microbial community diversity in coppercontaminated sites can be helpful to understand the impact of the metal on the microbiome and to design bioremediation processes. (AU)

• FAPESP's process: 12/06600-7 - Study on microbial activity in bioremediation of heavy metals in Amazon area
• Grantee: Ingrid Regina Avanzi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/11020-2 - Characterization of the bacterial community of a copper processing and identification of heavy metals resistance mechanism
• Grantee: Louise Hase Gracioso
• Support Opportunities: Scholarships in Brazil - Doctorate