Characterization of the bacterial community of a copper processing and identification of heavy metals resistance mechanism

• Grant number: 13/11020-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): November 01, 2013
• Effective date (End): June 30, 2017
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Elen Aquino Perpetuo
• Grantee: Louise Hase Gracioso
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 08/57708-7 - CEPEMA-USP, AP.TEM
• Associated scholarship(s): 15/18089-3 - Copper removal using a microbial consortium isolated from mining wastes, BE.EP.DR

Abstract

Despite great microorganisms importance of biosphere reserve maintenance, it is estimated only a small existing microbial species's fraction have been described and characterized. Through technological developments for the identification and classification of microorganisms, in recent decades, microbiologists have been discovered the enormous microbial diversity and correlating it with functioning of ecosystems, using molecular techniques. Environmental knowledge of microbial diversity constitutes an object of a great value to development of technology processes for bioremediation of degraded and contaminated compounds, because many species of micro-organisms are able to degrade or assist in the transformation of such substances. Heavy metals are one of the most relevant sources of environmental pollution since the industrial revolution, mainly because these metals are not degraded and, therefore, remain in the environment. In this situation, heavy metal-contaminated area maintains the selection process in native bacterial populations for a long period. Several micro-organisms have adapted successfully to presence of various metals by use of resistance mechanisms encoded by chromosomes, plasmids or transposons. The present work intends to study bacterial diversity of a region contaminated with copper, by proteomics analysis and study the most expressed proteins in the presence of high copper concentrations (elucidating the bacteria mechanisms of resistance to copper).

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRACIOSO, LOUISE H.; VIEIRA, PAULA B.; BALTAZAR, MARCELA P. G.; AVANZI, INGRID R.; KAROLSKI, BRUNO; NASCIMENTO, CLAUDIO A. O.; PERPETUO, ELEN A.. Removal of phenolic compounds from raw industrial wastewater by Achromobacter sp. isolated from a hydrocarbon-contaminated area. WATER AND ENVIRONMENT JOURNAL, v. 33, n. 1, p. 40-50, FEB 2019. (08/57708-7, 13/11020-2)

AVANZI, INGRID REGINA; GRACIOSO, LOUISE HASE; GALLUZZI BALTAZAR, MARCELA DOS PASSOS; KAROLSKI, BRUNO; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO. Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis. Environmental Science and Pollution Research, v. 24, n. 4, p. 3717-3726, FEB 2017. (12/06600-7, 13/11020-2)

GRACIOSO, LOUISE HASE; PENA-BAHAMONDE, JANIRE; KAROLSKI, BRUNO; BORREGO, BRUNA BACARO; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; HASHIGUCHI, HIROKI; JULIANO, MARIA APARECIDA; ROBLES HERNANDEZ, FRANCISCO C.; RODRIGUES, DEBORA FRIGI. Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper. SCIENCE ADVANCES, v. 7, n. 17, APR 2021. (15/18089-3, 18/22790-7, 13/11020-2, 13/50218-2, 18/13588-0)

GRACIOSO, LOUISE HASE; GALLUZZI BALTAZAR, MARCELA PASSOS; AVANZI, INGRID REGINA; KAROLSKI, BRUNO; OLLER NASCIMENTO, CLAUDIO AUGUSTO; PERPETUO, ELEN AQUINO. Analysis of copper response in Acinetobacter sp. by comparative proteomics. METALLOMICS, v. 11, n. 5, p. 949-958, MAY 1 2019. (12/06600-7, 13/50218-2, 13/11020-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GRACIOSO, Louise Hase. Characterization of bacterial community of a copper processing area and study of the mechanism of resistance to this metal.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.