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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rapid bacteria identification from environmental mining samples using MALDI-TOF MS analysis

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Avanzi, Ingrid Regina ; Gracioso, Louise Hase ; Galluzzi Baltazar, Marcela dos Passos ; Karolski, Bruno ; Perpetuo, Elen Aquino ; Oller do Nascimento, Claudio Augusto
Total Authors: 6
Document type: Journal article
Source: Environmental Science and Pollution Research; v. 24, n. 4, p. 3717-3726, FEB 2017.
Web of Science Citations: 8

Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level. Even though some results were ambiguous, accuracy can be improved by enhancing reference databases. Therefore, mass spectra analysis provides a reliable method to facilitate monitoring of the microbiota from copper-polluted sites. The understanding of the microbial community diversity in coppercontaminated sites can be helpful to understand the impact of the metal on the microbiome and to design bioremediation processes. (AU)

FAPESP's process: 12/06600-7 - Study on microbial activity in bioremediation of heavy metals in Amazon area
Grantee:Ingrid Regina Avanzi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/11020-2 - Characterization of the bacterial community of a copper processing and identification of heavy metals resistance mechanism
Grantee:Louise Hase Gracioso
Support type: Scholarships in Brazil - Doctorate