Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples

Autor(es)

Acquaro Junior, Vinicius Ricardo ; Domingues, Diego Soares ; Costa Queiroz, Maria Eugenia

• Tipo de documento: Artigo Científico
• Fonte: BIOANALYSIS; v. 9, n. 6, p. 555-568, MAR 2017.
• Citações Web of Science: 4

Resumo

Background: Polypharmacy is a common practice in schizophrenia. Consequently, therapeutic drug monitoring is usually adopted to maintain the concentrations of the drugs in the plasma within a targeted therapeutic range, to maximize therapeutic efficiency and to diminish adverse side effects. Methodology: This study reports on a column switching UHPLC-MS/MS method to determine psychotropic drugs in plasma samples simultaneously. Results: The method was linear from 0.025 to 1.25 ng ml(-1) with R-2 above 0.9950 and the lack of fit test (p > 0.05). The precision values presented coefficients of variation lower than 12%, and the relative standard error of the accuracy were lower than 14%. Conclusion: The column switching UHPLC-MS/MS method developed herein successfully determined drugs in schizophrenic patients' plasma samples for therapeutic drug monitoring. (AU)

• Processo FAPESP: 15/07619-1 - Cromatografia líquida acoplada à espectrometria de massas em tandem no modo column switching para análises de endocanabinóides e de fármacos em amostras biológicas
• Beneficiário: Maria Eugênia Queiroz Nassur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22140-1 - Cromatografia Líquida de Ultra Eficiência Bidimensional com Detecção por Espectrometria de Massas em Tandem (2D-UPLC-MS/MS) para determinação de fármacos em amostras de plasma de pacientes esquizofrênicos
• Beneficiário: Vinicius Ricardo Acquaro Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado