Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples

Author(s)

Acquaro Junior, Vinicius Ricardo ; Domingues, Diego Soares ; Costa Queiroz, Maria Eugenia

• Document type: Journal article
• Source: BIOANALYSIS; v. 9, n. 6, p. 555-568, MAR 2017.
• Web of Science Citations: 4

Abstract

Background: Polypharmacy is a common practice in schizophrenia. Consequently, therapeutic drug monitoring is usually adopted to maintain the concentrations of the drugs in the plasma within a targeted therapeutic range, to maximize therapeutic efficiency and to diminish adverse side effects. Methodology: This study reports on a column switching UHPLC-MS/MS method to determine psychotropic drugs in plasma samples simultaneously. Results: The method was linear from 0.025 to 1.25 ng ml(-1) with R-2 above 0.9950 and the lack of fit test (p > 0.05). The precision values presented coefficients of variation lower than 12%, and the relative standard error of the accuracy were lower than 14%. Conclusion: The column switching UHPLC-MS/MS method developed herein successfully determined drugs in schizophrenic patients' plasma samples for therapeutic drug monitoring. (AU)

• FAPESP's process: 14/22140-1 - Two-dimensional Ultra-Efficiency Liquid Chromatography with Detection by Tandem Mass Spectrometry (2D-UPLC-MS/MS) for determination of drugs in plasma samples from schizophrenic patients
• Grantee: Vinicius Ricardo Acquaro Junior
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
• Grantee: Maria Eugênia Queiroz Nassur
• Support type: Regular Research Grants