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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium

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Autor(es):
da Silva, Ronivaldo Rodrigues ; Goncalves de Oliveira, Lilian Caroline ; Juliano, Maria Aparecida ; Juliano, Luiz ; de Oliveira, Arthur H. C. ; Rosa, Jose C. ; Cabral, Hamilton
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 225, p. 45-54, JUN 15 2017.
Citações Web of Science: 19
Resumo

For a long time, proteolytic enzymes have been employed as key tools of industrial processes, especially in the dairy industry. In the present work, we used Phanerochaete chrysosporium for biochemical characterization and analysis of catalytic specificity of an aspartic peptidase. Our results revealed an aspartic peptidase with molecular mass similar to 38 kDa, maximal activity at pH 4.5 and 50 degrees C, and stability above 80% in the pH range of 3-8 and temperature up to 55 degrees C for 1 h. In a milk-clotting assay, this peptidase showed maximal milk clotting activity at 60-65 degrees C and maintenance of enzymatic activity above 80% in the presence of 20 mM CaCl2. In a specificity assay, we observed stronger restriction of catalysis at the S-1 subsite, with a preference for lysine, arginine, leucine, tyrosine, and phenylalanine residues. The restricted proteolysis and milk-clotting potential are attractive properties for the use in cheese production. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/06986-0 - Determinação da especificidade de peptidases isoladas de fungos usando peptídeos FRET como substratos
Beneficiário:Hamilton Cabral
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/24703-8 - Análises proteômicas de fungos filamentosos meso e termofílicos expostos aos fatores físico e químico
Beneficiário:Hamilton Cabral
Linha de fomento: Auxílio à Pesquisa - Regular