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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

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Autor(es):
Peters, Leila P. ; Carvalho, Giselle ; Vilhena, Milca B. ; Creste, Silvana ; Azevedo, Ricardo A. ; Monteiro-Vitorello, Claudia B.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: PLANTA; v. 245, n. 4, p. 749-764, APR 2017.
Citações Web of Science: 9
Resumo

Main Conclusion Smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. Sporisorium scitamineum is the causal agent of sugarcane smut disease. In this study, we characterized sugarcane reactive oxygen species (ROS) metabolism in response to the pathogen in smut-resistant and -susceptible genotypes. Sporisorium scitamineum teliospore germination and appressorium formation coincided with H2O2 accumulation in resistant plants. The superoxide dismutase (SOD) activity was not responsive in any of the genotypes; however, a higher number of isoenzymes were detected in resistant plants. In addition, related to resistance were lipid peroxidation, a decrease in catalase (CAT), and an increase in glutathione S-transferase (GST) activities and an earlier transcript accumulation of ROS marker genes (CAT3, CATA, CATB, GST31, GSTt3, and peroxidase 5-like). Furthermore, based on proteomic data, we suggested that the source of the increased hydrogen peroxide (H2O2) may be due to a protein of the class III peroxidase, which was inhibited in the susceptible genotype. H2O2 is sensed and probably transduced through overlapping systems related to ascorbate-glutathione and thioredoxin to influence signalling pathways, as revealed by the presence of thioredoxin h-type, ascorbate peroxidase, and guanine nucleotide-binding proteins in the infected resistant plants. Altogether, our data depicted the balance of the oxidative burst and antioxidant enzyme activity in the outcome of this interaction. (AU)

Processo FAPESP: 09/54676-0 - Estresse oxidativo induzido por metais: novas abordagens
Beneficiário:Ricardo Antunes de Azevedo
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/07112-4 - Ligando os fatos sobre a interação molecular da cana-de-açúcar e o fungo biotrófico patogênico Sporisorium scitamineum, causador do carvão da cana
Beneficiário:Claudia Barros Monteiro Vitorello
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/15014-7 - Análise genética e bioquímica da sinalização de espécies reativas de oxigênio na interação cana-de-açúcar e Sporisorium scitamineum
Beneficiário:Leila Priscila Peters
Linha de fomento: Bolsas no Brasil - Doutorado