Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes

Author(s)

Peters, Leila P. ; Carvalho, Giselle ; Vilhena, Milca B. ; Creste, Silvana ; Azevedo, Ricardo A. ; Monteiro-Vitorello, Claudia B.

• Document type: Journal article
• Source: PLANTA; v. 245, n. 4, p. 749-764, APR 2017.
• Web of Science Citations: 9

Abstract

Main Conclusion Smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. Sporisorium scitamineum is the causal agent of sugarcane smut disease. In this study, we characterized sugarcane reactive oxygen species (ROS) metabolism in response to the pathogen in smut-resistant and -susceptible genotypes. Sporisorium scitamineum teliospore germination and appressorium formation coincided with H2O2 accumulation in resistant plants. The superoxide dismutase (SOD) activity was not responsive in any of the genotypes; however, a higher number of isoenzymes were detected in resistant plants. In addition, related to resistance were lipid peroxidation, a decrease in catalase (CAT), and an increase in glutathione S-transferase (GST) activities and an earlier transcript accumulation of ROS marker genes (CAT3, CATA, CATB, GST31, GSTt3, and peroxidase 5-like). Furthermore, based on proteomic data, we suggested that the source of the increased hydrogen peroxide (H2O2) may be due to a protein of the class III peroxidase, which was inhibited in the susceptible genotype. H2O2 is sensed and probably transduced through overlapping systems related to ascorbate-glutathione and thioredoxin to influence signalling pathways, as revealed by the presence of thioredoxin h-type, ascorbate peroxidase, and guanine nucleotide-binding proteins in the infected resistant plants. Altogether, our data depicted the balance of the oxidative burst and antioxidant enzyme activity in the outcome of this interaction. (AU)

• FAPESP's process: 13/15014-7 - Genetic and biochemical analysis of signaling of reactive oxygen species in the interaction sugarcane and Sporisorium scitamineum
• Grantee: Leila Priscila Peters
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/54676-0 - Oxidative stress induced by metals: new approaches
• Grantee: Ricardo Antunes de Azevedo
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/07112-4 - Connecting the dots to understand sugarcane smut: systems biology of the sugarcane-Sporisorium scitamineum molecular interaction
• Grantee: Claudia Barros Monteiro Vitorello
• Support Opportunities: Regular Research Grants