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Genetic and biochemical analysis of signaling of reactive oxygen species in the interaction sugarcane and Sporisorium scitamineum

Grant number: 13/15014-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2013
Effective date (End): July 31, 2016
Field of knowledge:Agronomical Sciences - Agronomy
Principal researcher:Claudia Barros Monteiro Vitorello
Grantee:Leila Priscila Peters
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Sugarcane is a crop cultivated in more than 100 tropical countries and in Brazil it has contributed to generation of renewable fuel and less pollutants, as well as to economic and social development. However, its productivity may be affected by several diseases, such as the smut caused by Sporisorium scitamineum fungi. The colonization of this fungus in sugarcane depends on environmental conditions, variety and interaction with the pathogen. In general, pathogens induce specific pathways of signal transduction resulting in plant susceptibility or resistance. This process, in the early stages of infection, involves the production of reactive oxygen species (ROS) in a phenomenon known as oxidative burst. ROS can act directly against pathogens and act as second messengers in the activation of plant defense responses. However, high concentrations of ROS can damage plant biomolecules itself. To understand how the oxidative burst caused by ROS triggers the success or failure of this interaction, this project aims to identify genes and proteins modulated by ROS in the initial interaction of S. scitamineum and sugarcane, using varieties with different levels of resistance to S. scitamineum. For this, we will use the following strategies: i) establishment of the times in the oxidative burst that occurs in the course of the interaction (time course) by biochemical markers, ii) assessment of expression of genes related to oxidative burst (time course) via real-time qPCR and iii) identification of differentially expressed proteins in the times established in the time course through analysis of proteomics. Thus, with these results we expect to better understand the role of ROS and the antioxidant system in the context of network defense response against this pathogen.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PETERS, L. P.; TEIXEIRA-SILVA, N. S.; BINI, A. P.; SILVA, M. M. L.; MORAES, N.; CRESTANA, G. S.; CRESTE, S.; AZEVEDO, R. A.; CARVALHO, G.; MONTEIRO-VITORELLO, C. B. Differential responses of genes and enzymes associated with ROS protective responses in the sugarcane smut fungus. FUNGAL BIOLOGY, v. 124, n. 12, p. 1039-1051, DEC 2020. Web of Science Citations: 1.
PETERS, LEILA P.; CARVALHO, GISELLE; VILHENA, MILCA B.; CRESTE, SILVANA; AZEVEDO, RICARDO A.; MONTEIRO-VITORELLO, CLAUDIA B. Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes. PLANTA, v. 245, n. 4, p. 749-764, APR 2017. Web of Science Citations: 9.
TANIGUTI, LUCAS M.; SCHAKER, PATRICIA D. C.; BENEVENUTO, JULIANA; PETERS, LEILA P.; CARVALHO, GISELLE; PALHARES, ALESSANDRA; QUECINE, MARIA C.; NUNES, FILIPE R. S.; KMIT, MARIA C. P.; WAI, ALVAN; HAUSNER, GEORG; AITKEN, KAREN S.; BERKMAN, PAUL J.; FRASER, JAMES A.; MOOLHUIJZEN, PAULA M.; COUTINHO, LUIZ L.; CRESTE, SILVANA; VIEIRA, MARIA L. C.; KITAJIMA, JOAO P.; MONTEIRO-VITORELLO, CLAUDIA B. Complete Genome Sequence of Sporisorium scitamineum and Biotrophic Interaction Transcriptome with Sugarcane. PLoS One, v. 10, n. 6 JUN 12 2015. Web of Science Citations: 38.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PETERS, Leila Priscila. A more detailed view of reactive oxygen species metabolism in the sugarcane and Sporisorium scitamineum interaction. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz Piracicaba.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.