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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential transcript profile of inhibitors with potential anti-venom role in the liver of juvenile and adult Bothrops jararaca snake

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Autor(es):
Gomes, Cicera Maria ; de Morais-Zani, Karen ; Lu, Stephen ; Buarque, Diego de Souza ; Cardoso Braz, Gloria Regina ; Grego, Kathleen Fernandes ; Tanaka, Aparecida Sadae ; Tanaka-Azevedo, Anita Mitico
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: PeerJ; v. 5, APR 27 2017.
Citações Web of Science: 2
Resumo

Background. Snakes belonging to the Bothrops genus are vastly distributed in Central and South America and are responsible for most cases of reported snake bites in Latin America. The clinical manifestations of the envenomation caused by this genus are due to three major activities-proteolytic, hemorrhagic and coagulant-mediated by metalloproteinases, serine proteinases, phospholipases A(2) and other toxic compounds present in snake venom. Interestingly, it was observed that snakes are resistant to the toxic effects of its own and other snake's venoms. This natural immunity may occur due the absence of toxin target or the presence of molecules in the snake plasma able to neutralize such toxins. Methods. In order to identify anti-venom molecules, we construct a cDNA library from the liver of B. jararaca snakes. Moreover, we analyzed the expression profile of four molecules-the already known anti-hemorrhagic factor Bj46a, one gamma-phospholipase A(2) inhibitor, one inter-alpha inhibitor and one C1 plasma protease inhibitor-in the liver of juvenile and adult snakes by qPCR. Results. The results revealed a 30-fold increase of gamma-phospholipase A(2) inhibitor and a minor increase of the inter-alpha inhibitor (5-fold) and of the C1 inhibitor (3-fold) in adults. However, the Bj46a factor seems to be equally transcribed in adults and juveniles. Discussion. The results suggest the up-regulation of different inhibitors observed in the adult snakes might be a physiological adaptation to the recurrent contact with their own and even other snake's venoms throughout its lifespan. This is the first comparative analysis of ontogenetic variation of expression profiles of plasmatic proteins with potential anti-venom activities of the venomous snake B. jararaca. Furthermore, the present data contributes to the understanding of the natural resistance described in these snakes. (AU)

Processo FAPESP: 14/11108-0 - Estudo comparativo da composição das biomoléculas dos venenos das serpentes Bothrops jararaca e Crotalus durissus terrificus nascidas e mantidas no Instituto Butantan e recém chegadas da natureza
Beneficiário:Karen de Morais Zani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/03657-8 - Inibidores e proteases de ectoparasitas: relação de estrutura-função e identificação do papel dessas moléculas na interação de vetores de doenças e seus agentes etiológicos
Beneficiário:Aparecida Sadae Tanaka
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/05357-4 - Estudo comparativo do veneno botrópico de referência em relação ao veneno das serpentes Bothrops jararaca nascidas em cativeiro no Laboratório de Herpetologia do Instituto Butantan
Beneficiário:Anita Mitico Tanaka-Azevedo
Linha de fomento: Auxílio à Pesquisa - Regular