Identification of Long Noncoding RNAs Deregulated in Papillary Thyroid Cancer and Correlated with BRAF(V600E) Mutation by Bioinformatics Integrative Analysis

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Goedert, Lucas ; Placa, Jessica Rodrigues ; Fuziwara, Cesar Seigi ; Rosa Machado, Maiaro Cabral ; Placa, Desiree Rodrigues ; Almeida, Palloma Porto ; Sanches, Talita Perez ; dos Santos, Jair Figueredo ; Corveloni, Amanda Cristina ; Gomes Pereira, Illy Enne ; de Castro, Marcela Motta ; Kimura, Edna Teruko ; Silva, Jr., Wilson Araujo ; Espreafico, Enilza Maria

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, MAY 10 2017.
• Citações Web of Science: 9

Resumo

Papillary Thyroid Cancer (PTC) is an endocrine malignancy in which BRAF(V600E) oncogenic mutation induces the most aggressive phenotype. In this way, considering that lncRNAs are arising as key players in oncogenesis, it is of high interest the identification of BRAF(V600E)-associated long noncoding RNAs, which can provide possible candidates for secondary mechanisms of BRAF-induced malignancy in PTC. In this study, we identified differentially expressed lncRNAs correlated with BRAF(V600E) in PTC and, also, extended the cohort of paired normal and PTC samples to more accurately identify differentially expressed lncRNAs between these conditions. Indirectly validated targets of the differentially expressed lncRNAs in PTC compared to matched normal samples demonstrated an involvement in surface receptors responsible for signal transduction and cell adhesion, as well as, regulation of cell death, proliferation and apoptosis. Targets of BRAF(V600E)-correlated lncRNAs are mainly involved in calcium signaling pathway, ECM-receptor interaction and MAPK pathway. In summary, our study provides candidate lncRNAs that can be either used for future studies related to diagnosis/prognosis or as targets for PTC management. (AU)

• Processo FAPESP: 14/50521-0 - Regulação transcricional do microRNA miR-17-92 no câncer de tiroide agressivo
• Beneficiário: Cesar Seigi Fuziwara
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/13396-5 - Drosophila melanogaster como modelo para estudo de câncer e doenças degenerativas: estudo funcional do gene DmelEMC1 e as interações genéticas com outros membros do complexo EMC
• Beneficiário: Maiaro Cabral Rosa Machado
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/07726-0 - Caracterização genômica e funcional de genes de expressão restrita a melanoma (RMELs)
• Beneficiário: Lucas Goedert
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/18189-5 - Investigação de um possível circuito funcional envolvendo microRNAs, MITF, MYO5A/DLC2, Rab27a e conexões com a cascata de invasão e metástase do melanoma
• Beneficiário: Enilza Maria Espreafico
• Linha de fomento: Auxílio à Pesquisa - Regular