Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil

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Nunes-Neto, Joaquim Pinto ; de Souza, William Marciel ; Acrani, Gustavo Olszanski ; Romeiro, Marilia Farignoli ; Fumagalli, MarcilioJorge ; Vieira, Luiz Carlos ; de Almeida Medeiros, Daniele Barbosa ; Lima, Juliana Abreu ; Silva de Lima, Clayton Pereira ; Cardoso, Jedson Ferreira ; Moraes Figueiredo, Luiz Tadeu ; da Silva, Sandro Patroca ; Tesh, Robert ; Teixeira Nunes, Marcio Roberto ; da Costa Vasconcelos, Pedro Fernando

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GENERAL VIROLOGY; v. 98, n. 4, p. 585-594, APR 2017.
• Citações Web of Science: 4

Resumo

The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaruantigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses. (AU)

• Processo FAPESP: 12/24150-9 - Pesquisa de vírus em roedores silvestres, mosquitos e carrapatos
• Beneficiário: William Marciel de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/01414-1 - Produção de peptídeos recombinantes virais antigênicos e desenvolvimento de métodos sorológicos para diagnóstico de infecções pelos vírus Chikungunya, Mayaro e Zika
• Beneficiário: Marcilio Jorge Fumagalli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/20851-8 - Estudo sobre infecções por Alphavirus e Flavivirus em amostras de Banco de Sangue
• Beneficiário: Marilia Farignoli Romeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/05778-5 - Análises de bioinformática de dados de metagenômica viral de animais do Brasil e Antártida
• Beneficiário: William Marciel de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/14929-1 - Detecção de arbovírus e outros vírus de RNA por RT-PCR em tempo real e estudo do viroma
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/15079-4 - Pesquisa de virus de RNA em amostras séricas e artropodes
• Beneficiário: Luiz Carlos Vieira
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 14/02438-6 - Estudos com Bunyaviridae causadores de doença
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Modalidade de apoio: Auxílio à Pesquisa - Temático