Characterization of the Bujaru, frijoles and Tapara antigenic complexes into the sandfly fever group and two unclassified phleboviruses from Brazil

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Nunes-Neto, Joaquim Pinto ; de Souza, William Marciel ; Acrani, Gustavo Olszanski ; Romeiro, Marilia Farignoli ; Fumagalli, MarcilioJorge ; Vieira, Luiz Carlos ; de Almeida Medeiros, Daniele Barbosa ; Lima, Juliana Abreu ; Silva de Lima, Clayton Pereira ; Cardoso, Jedson Ferreira ; Moraes Figueiredo, Luiz Tadeu ; da Silva, Sandro Patroca ; Tesh, Robert ; Teixeira Nunes, Marcio Roberto ; da Costa Vasconcelos, Pedro Fernando

• Document type: Journal article
• Source: JOURNAL OF GENERAL VIROLOGY; v. 98, n. 4, p. 585-594, APR 2017.
• Web of Science Citations: 3

Abstract

The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaruantigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses. (AU)

• FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
• Grantee: William Marciel de Souza
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/01414-1 - Production of recombinant viral antigenic peptides and development of methods for serological diagnosis of infections by Chikungunya, Mayaro and Zika viruses
• Grantee: Marcilio Jorge Fumagalli
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 13/14929-1 - Detection of arbovirus and other RNA viruses by real time RT-PCR and study of the viroma
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support type: Regular Research Grants
• FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
• Grantee: Marilia Farignoli Romeiro
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/02438-6 - Studies with Bunyaviridae that produce human disease
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 15/05778-5 - Bioinformatics analysis of data of viral metagenomic of animals from Brazil and Antarctica
• Grantee: William Marciel de Souza
• Support type: Scholarships abroad - Research Internship - Doctorate