| Texto completo | |
| Autor(es): |
Abriata, Juliana Palma
;
Eloy, Josimar O.
;
Riul, Thalita Bachelli
;
Campos, Patricia Mazureki
;
Baruffi, Marcelo Dias
;
Marchetti, Juliana Maldonado
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Materials Science & Engineering C-Materials for Biological Applications; v. 77, p. 1196-1203, AUG 1 2017. |
| Citações Web of Science: | 6 |
| Resumo | |
Despite affecting millions of people worldwide, Chagas disease is still neglected by the academia and industry and the therapeutic option available, benznidazole, presents limited efficacy and side effects. Within this context, ursolic acid may serve as an option for treatment, however has low bioavailability, which can be enhanced through the encapsulation in polymeric nanoparticles. Therefore, herein we developed ursolic acid-loaded nano particles with poly-c-caprolactone by the nanoprecipitation method and characterized them for particle size, zeta potential, polydispersity, encapsulation efficiency, morphology by scanning electron microscopy and thermal behavior by differential scanning calorimetry. Results indicated that an appropriate ratio of organic phase/aqueous phase and polymer/drug is necessary to produce smaller particles, with low polydispersity, negative zeta potential and high drug encapsulation efficiency. In vitro studies indicated the safety of the formulation against fibroblast culture and its efficacy in killing T. cruzi. Very importantly, the in vivo study revealed that the ursolic acid loaded nanopartide is as potent as the benznidazole group to control parasitemia, which could be attributed to improved bioavailability of the encapsulated drug. Finally, the toxicity evaluation showed that while benznidazole group caused liver toxicity, the nanoparticles were safe, indicating that this formulation is promising for future evaluation. (C) 2017 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 12/01515-1 - Potencialidade do uso de sistemas nanoestruturados contendo ácido ursólico para a otimização da terapia da Doença de Chagas |
| Beneficiário: | JULIANA PALMA ABRIATA |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |