| Texto completo | |
| Autor(es): |
Nakajima, Vania Mayumi
;
Moala, Tais
;
Caria, Cintia Rabelo e Paiva
;
Moura, Carolina Soares
;
Amaya-Farfan, Jaime
;
Gambero, Alessandra
;
Macedo, Gabriela Alves
;
Macedo, Juliana Alves
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Food Research International; v. 97, p. 37-44, JUL 2017. |
| Citações Web of Science: | 4 |
| Resumo | |
Chronic non-communicable diseases such as obesity are preceded by increased macrophage infiltration in adipose tissue and greater secretion of pro-inflammatory cytokines. We evaluated the anti-inflammatory potential of Biotransformed extract, and two control extracts: In Natura and Autoclaved. The assays were performed using a cellular model with RAW264.7, 3T3-L1 cells, and RAW264.7 and 313-L1 co-culture. The innovation of the study was the use of Biotransformed extract, a unique phenolic extract of a bioprocessed citrus residue. LPS stimulated RAW264.7 cells treated with the Biotransformed extract exhibited lower secretion of TNF-alpha and NO and lower protein expression of NE kappa B. In RAW264.7 and 3T3-L1 co-culture, treatment with 1.0 mg/mL of the Biotransformed extract reduced secretion of TNF-alpha(30.7%) and IL-6 (43.4%). Still, the Biotransformed extract caused higher increase in adiponectin in relation to control extracts. When the co-culture received a LPS stimulus, the Autoclaved extract at 1.0 mg/mL reduced IL-6 and TNE-alpha concentrations, and raised adiponectin. However, it was noteworthy that the Biotransformed extract was also able to significantly reduce IL-6 concentration while the Natural extract was not. The Biotransformed citrus extract evaluated in this study showed anti-inflammatory activity in macrophages and in co-culture, indicating that bioprocess of citrus residue can contribute to new product development with anti-inflammatory potential. (C) 2017 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 15/04555-2 - Desenvolvimento de processos biológicos para obtenção de extratos ricos em compostos fenólicos a partir de resíduos da indústria de cítricos e avaliação do potencial bioativo dos extratos |
| Beneficiário: | Gabriela Alves Macedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/06494-5 - Impacto metabólico da proteína dietética do soro do leite, seus peptídeos e aminoácidos nas heat-shock proteins (HSPs) e parâmetros associados em ratos |
| Beneficiário: | Jaime Amaya Farfan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |