Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biotransformed citrus extract as a source of anti-inflammatory polyphenols: Effects in macrophages and adipocytes

Full text
Author(s):
Nakajima, Vania Mayumi ; Moala, Tais ; Caria, Cintia Rabelo e Paiva ; Moura, Carolina Soares ; Amaya-Farfan, Jaime ; Gambero, Alessandra ; Macedo, Gabriela Alves ; Macedo, Juliana Alves
Total Authors: 8
Document type: Journal article
Source: Food Research International; v. 97, p. 37-44, JUL 2017.
Web of Science Citations: 4
Abstract

Chronic non-communicable diseases such as obesity are preceded by increased macrophage infiltration in adipose tissue and greater secretion of pro-inflammatory cytokines. We evaluated the anti-inflammatory potential of Biotransformed extract, and two control extracts: In Natura and Autoclaved. The assays were performed using a cellular model with RAW264.7, 3T3-L1 cells, and RAW264.7 and 313-L1 co-culture. The innovation of the study was the use of Biotransformed extract, a unique phenolic extract of a bioprocessed citrus residue. LPS stimulated RAW264.7 cells treated with the Biotransformed extract exhibited lower secretion of TNF-alpha and NO and lower protein expression of NE kappa B. In RAW264.7 and 3T3-L1 co-culture, treatment with 1.0 mg/mL of the Biotransformed extract reduced secretion of TNF-alpha(30.7%) and IL-6 (43.4%). Still, the Biotransformed extract caused higher increase in adiponectin in relation to control extracts. When the co-culture received a LPS stimulus, the Autoclaved extract at 1.0 mg/mL reduced IL-6 and TNE-alpha concentrations, and raised adiponectin. However, it was noteworthy that the Biotransformed extract was also able to significantly reduce IL-6 concentration while the Natural extract was not. The Biotransformed citrus extract evaluated in this study showed anti-inflammatory activity in macrophages and in co-culture, indicating that bioprocess of citrus residue can contribute to new product development with anti-inflammatory potential. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/06494-5 - Metabolic impact of dietary whey proteins, peptides and amino acids thereof on the heat-shock proteins (HSPs) and associated parameters in rats
Grantee:Jaime Amaya Farfan
Support type: Regular Research Grants
FAPESP's process: 15/04555-2 - Biological process development of rich phenolics extracts from citrus waste industry and evaluation of bioactivity from extracts
Grantee:Gabriela Alves Macedo
Support type: Regular Research Grants