Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

Autor(es)

Argandona Valdez, Remberto Marcelo ; Duque, Cristiane ; Caiaffa, Karina Sampaio ; dos Santos, Vanessa Rodrigues ; de Aguiar Loesch, Maria Luiza ; Colombo, Natalia Helena ; Arthur, Rodrigo Alex ; Negrini, Thais de Cassia ; Gomes Boriollo, Marcelo Fabiano ; Botazzo Delbem, Alberto Carlos

• Tipo de documento: Artigo Científico
• Fonte: BMC ORAL HEALTH; v. 17, JUL 14 2017.
• Citações Web of Science: 6

Resumo

Background: Early childhood caries (ECC) is an aggressive condition that can affect teeth of young children. This study aimed to evaluate genotypic diversity and phenotypic traits of S. mutans isolated from dental biofilms of children with different caries status in comparison with caries free (CF) children. Methods: Streptococcus mutans strains were isolated from supragingival biofilm samples of CF, ECC and severe-ECC (S-ECC) children and genotyped by arbitrary-primer polymerase chain reaction - AP-PCR. S. mutans genotypes were tested for their ability to reduce the suspension pH through glycolysis, to tolerate extreme acid challenge and by their ability to form biofilm. Response variables were analyzed by ANOVA/Tukey or Kruskal-Wallis/Mann-Whitney tests at a 5% of significance. Results: There was an increase in the prevalence of Streptococcus mutans in biofilms with the severity of dental caries. No differences in genotypic diversity and in acidogenicity of genotypes were found among CF, ECC and S-ECC children. S mutans strains with genotypes more characteristic for ECC and S-ECC children formed more biofilms than those identified in CF children. The strains isolated from S-ECC children were highly acid tolerant. Conclusion: Although S. mutans genotypic diversity was similar among the groups of children, phenotypic traits of S. mutans, especially the acid tolerance response, could explain the severity of early childhood caries. (AU)

• Processo FAPESP: 14/02072-1 - Avaliação do potencial cariogênico in vitro de espécies de bifidobactérias
• Beneficiário: Vanessa Rodrigues dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica