Monoamine oxidase and transaminase screening: biotransformation of 2-methyl-6-alkylpiperidines by Neopestalotiopsis sp CBMAI 2030

Autor(es)

Costa, Jonas Henrique ; da Costa, Bruna Zucoloto ; de Angelis, Derlene Attili ; Marsaioli, Anita Jocelyne

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 101, n. 15, p. 6061-6070, AUG 2017.
• Citações Web of Science: 2

Resumo

High-throughput screening detected transaminases (TAs) and monoamine oxidases (MAOs) in fungi by applying a fluorogenic probe. Strains F026, F037, F041, F053, and F057 showed the highest enzymatic conversions (31, 60, 30, 40, and 32%, respectively) and where evaluated for their ability to transform piperidines. Strain F053 (Neopestalotiopsis sp. CBMAI 2030) revealed unusual enzymatic activity to deracemize 2-methyl-6-alkylpiperidines. Neopestalotiopsis sp. CBMAI 2030 was capable to convert 2-methyl-6-propylpiperidine, 2-methyl-6-butylpiperidine, and 2-methyl-6-pentylpiperidine in piperideine with 11, 14, and 24% conversion, respectively. The activity was enhanced by cultivating the fungus with 2-methyl-6-pentylpiperidine (38% conversion and 73% ee). (AU)

• Processo FAPESP: 14/22967-3 - Síntese de Alcalóides Piperidínicos e Pirrolidínicos em Sistemas Multienzimáticos
• Beneficiário: Bruna Zucoloto da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/51278-0 - Potencial enzimático de minas de ferro, cobre e ouro
• Beneficiário: Anita Jocelyne Marsaioli
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE