Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monoamine oxidase and transaminase screening: biotransformation of 2-methyl-6-alkylpiperidines by Neopestalotiopsis sp CBMAI 2030

Full text
Author(s):
Costa, Jonas Henrique ; da Costa, Bruna Zucoloto ; de Angelis, Derlene Attili ; Marsaioli, Anita Jocelyne
Total Authors: 4
Document type: Journal article
Source: Applied Microbiology and Biotechnology; v. 101, n. 15, p. 6061-6070, AUG 2017.
Web of Science Citations: 2
Abstract

High-throughput screening detected transaminases (TAs) and monoamine oxidases (MAOs) in fungi by applying a fluorogenic probe. Strains F026, F037, F041, F053, and F057 showed the highest enzymatic conversions (31, 60, 30, 40, and 32%, respectively) and where evaluated for their ability to transform piperidines. Strain F053 (Neopestalotiopsis sp. CBMAI 2030) revealed unusual enzymatic activity to deracemize 2-methyl-6-alkylpiperidines. Neopestalotiopsis sp. CBMAI 2030 was capable to convert 2-methyl-6-propylpiperidine, 2-methyl-6-butylpiperidine, and 2-methyl-6-pentylpiperidine in piperideine with 11, 14, and 24% conversion, respectively. The activity was enhanced by cultivating the fungus with 2-methyl-6-pentylpiperidine (38% conversion and 73% ee). (AU)

FAPESP's process: 14/22967-3 - Piperidine and Pyrrolidine Alkaloids Synthesis in Multi-enzymatic systems
Grantee:Bruna Zucoloto da Costa
Support Opportunities: Scholarships in Brazil - Post-Doctoral