Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

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Galatro, Thais F. ; Holtman, Inge R. ; Lerario, Antonio M. ; Vainchtein, Ilia D. ; Brouwer, Nieske ; Sola, Paula R. ; Veras, Mariana M. ; Pereira, Tulio F. ; Leite, Renata E. P. ; Moller, Thomas ; Wes, Paul D. ; Sogayar, Mari C. ; Laman, Jon D. ; den Dunnen, Wilfred ; Pasqualucci, Carlos A. ; Oba-Shinjo, Sueli M. ; Boddeke, Erik W. G. M. ; Marie, Suely K. N. ; Eggen, Bart J. L.

• Tipo de documento: Artigo Científico
• Fonte: NATURE NEUROSCIENCE; v. 20, n. 8, p. 1162+, AUG 2017.
• Citações Web of Science: 127

Resumo

Microglia are essential for CNS homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. Overall, genes expressed by human microglia were similar to those in mouse, including established microglial genes CX3CR1, P2RY12 and ITGAM (CD11B). However, a number of immune genes, not identified as part of the mouse microglial signature, were abundantly expressed in human microglia, including TLR, F-c gamma and SIGLEC receptors, as well as TAL1 and IFI16, regulators of proliferation and cell cycle. Age-associated changes in human microglia were enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression and actin (dis)assembly. Limited overlap was observed in microglial genes regulated during aging between mice and humans, indicating that human and mouse microglia age differently. (AU)

• Processo FAPESP: 13/02162-8 - Patogênese molecular e caracterização de doenças monogênicas do desenvolvimento: um caminho para a medicina translacional
• Beneficiário: Berenice Bilharinho de Mendonça
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/06315-3 - Papel da ativação da micróglia em astrocitomas humanos
• Beneficiário: Suely Kazue Nagahashi Marie
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07704-3 - Papel da ativação da micróglia em astrocitomas humanos
• Beneficiário: Thais Fernanda de Almeida Galatro
• Modalidade de apoio: Bolsas no Brasil - Doutorado