Characterization of Corynebacterium diphtheriae, C. confusum and C-amycolatum isolated from sows with genitourinary infection

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Poor, Andre P. ; Moreno, Luisa Z. ; Matajira, Carlos E. C. ; Parra, Beatriz M. ; Gomes, Vasco T. M. ; Silva, Ana Paula S. ; Dutra, Mauricio C. ; Christ, Ana Paula G. ; Barbosa, Mikaela R. F. ; Sato, Maria Ines Z. ; Moreno, Andrea M.

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 207, p. 149-152, AUG 2017.
• Citações Web of Science: 2

Resumo

Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing. All porcine C. diphtheriae strains were further characterized as non-toxigenic (tox(-)). SE-AFLP genotyping was also performed and enabled not only Corynebacterium species differentiation but also the assessment of C. amycolatum genetic heterogeneity. All studied porcine Corynebacterium strains presented alarming resistance profiles with high MIC values for macrolides/lincosamide, tetracyclines and quinolones, which can be related with high usage in both veterinary and human medicine. Isolation of zoonotic Corynebacterium species from commercial swine is important for assessing the potential zoonotic risk for farmers and further implication for both human and animal treatment. (AU)

• Processo FAPESP: 15/26159-1 - Análise comparativa de genomas de estirpes de Streptococcus suis isoladas de suínos no Brasil
• Beneficiário: Carlos Emilio Cabrera Matajira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/26412-9 - Caracterização de estirpes de Corynebacterium spp. isoladas de infecção urinária de suínos
• Beneficiário: André Pegoraro Poor
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/16946-0 - Caracterização fenotípica e genotípica de cepas de Salmonella entérica isoladas de carne suína no município de São Paulo.
• Beneficiário: Vasco Túlio de Moura Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado