Characterization of Corynebacterium diphtheriae, C. confusum and C-amycolatum isolated from sows with genitourinary infection

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Poor, Andre P. ; Moreno, Luisa Z. ; Matajira, Carlos E. C. ; Parra, Beatriz M. ; Gomes, Vasco T. M. ; Silva, Ana Paula S. ; Dutra, Mauricio C. ; Christ, Ana Paula G. ; Barbosa, Mikaela R. F. ; Sato, Maria Ines Z. ; Moreno, Andrea M.

• Document type: Journal article
• Source: Veterinary Microbiology; v. 207, p. 149-152, AUG 2017.
• Web of Science Citations: 2

Abstract

Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing. All porcine C. diphtheriae strains were further characterized as non-toxigenic (tox(-)). SE-AFLP genotyping was also performed and enabled not only Corynebacterium species differentiation but also the assessment of C. amycolatum genetic heterogeneity. All studied porcine Corynebacterium strains presented alarming resistance profiles with high MIC values for macrolides/lincosamide, tetracyclines and quinolones, which can be related with high usage in both veterinary and human medicine. Isolation of zoonotic Corynebacterium species from commercial swine is important for assessing the potential zoonotic risk for farmers and further implication for both human and animal treatment. (AU)

• FAPESP's process: 15/26159-1 - Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil
• Grantee: Carlos Emilio Cabrera Matajira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/26412-9 - Characterization of Corynebacterium spp. strains isolated from urinary infection in pigs
• Grantee: André Pegoraro Poor
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by pulsed field gel electrophoresis (PFGE) typing and comparative genomic analysis.
• Grantee: Luisa Zanolli Moreno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/16946-0 - Phenotypic and genotypic characterization of strains of Salmonella enterica isolated from pork in São Paulo.
• Grantee: Vasco Túlio de Moura Gomes
• Support Opportunities: Scholarships in Brazil - Doctorate