| Texto completo | |
| Autor(es): Mostrar menos - |
Sobroza, Anderson O.
;
Pillat, Micheli M.
;
Dorneles, Guilherme L.
;
Machado, Marcia S. N.
;
Silva, Cassia B.
;
Petry, Leticia
;
Schafer, Andressa
;
Franca, Raqueli T.
;
de Andrade, Cinthia M.
;
Antoniazzi, Alfredo Q.
;
Tonin, Alexandre A.
;
Lopes, Sonia T. A.
Número total de Autores: 12
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| Tipo de documento: | Artigo Científico |
| Fonte: | Microbial Pathogenesis; v. 110, p. 450-456, SEP 2017. |
| Citações Web of Science: | 0 |
| Resumo | |
The aim of this study was to evaluate the behavior of erythrocyte and platelet, as immunological markers, as well as evaluate the involvement of these factors in hemolytic and hemorrhagic reactions in hamsters experimentally infected by Leptospira interrogans Serovar Canicola. Our experimental design was composed by two randomized groups: Infected Group (IG) (n = 12) and control group (CG) (n = 6). Ninety-six hours after the inoculation, the presence of immunoglobulins (IgG and IgM) and complement 0 levels, related to erythrocytes and platelets, was assessed. Platelet's microparticles marked by CD61, reticulocytes and reticulated platelets were also quantified. Additionally, fibrinogen, prothrombin time, partially activated thromboplastin time and sera levels of IgG and IgM were assessed. Our results showed that levels of platelet decreased in IG (P < 0.001); as well as, there was presence of IgG and C3 associated with erythrocyte surface in the infected animals (P < 0.01, P < 0.05, respectively). Levels of prothrombin time and Activated Partial Thromboplastin Time were increased, while fibrinogen level was decreased (P < 0.01) in IG. CD61 microparticles were higher (P < 0.05) in IG due to platelet activation. Thus, it was established a positive correlation (P < 0.01) between platelets count and fibrinogen (Figure 3, R = 0.84, P < 0.001). Therefore, the platelet consumption component was preponderant in relation to autoimmune causes. Finally, regarding the erythrocytes, the autoimmune component played an important role, did not causing hemolytic reaction in this acute experimental time. (C) 2017 Published by Elsevier Ltd. (AU) | |
| Processo FAPESP: | 15/19478-3 - Papel do receptor B2 de cininas na patogênese e progressão da doença de Alzheimer família experimental: da neurogênese e resposta imune à cognição |
| Beneficiário: | Micheli Mainardi Pillat |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |