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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anti-erythrocyte IgG in hamsters with acute experimental infection by Leptospira interrogans serovar Canicola

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Sobroza, Anderson O. ; Pillat, Micheli M. ; Dorneles, Guilherme L. ; Machado, Marcia S. N. ; Silva, Cassia B. ; Petry, Leticia ; Schafer, Andressa ; Franca, Raqueli T. ; de Andrade, Cinthia M. ; Antoniazzi, Alfredo Q. ; Tonin, Alexandre A. ; Lopes, Sonia T. A.
Total Authors: 12
Document type: Journal article
Source: Microbial Pathogenesis; v. 110, p. 450-456, SEP 2017.
Web of Science Citations: 0

The aim of this study was to evaluate the behavior of erythrocyte and platelet, as immunological markers, as well as evaluate the involvement of these factors in hemolytic and hemorrhagic reactions in hamsters experimentally infected by Leptospira interrogans Serovar Canicola. Our experimental design was composed by two randomized groups: Infected Group (IG) (n = 12) and control group (CG) (n = 6). Ninety-six hours after the inoculation, the presence of immunoglobulins (IgG and IgM) and complement 0 levels, related to erythrocytes and platelets, was assessed. Platelet's microparticles marked by CD61, reticulocytes and reticulated platelets were also quantified. Additionally, fibrinogen, prothrombin time, partially activated thromboplastin time and sera levels of IgG and IgM were assessed. Our results showed that levels of platelet decreased in IG (P < 0.001); as well as, there was presence of IgG and C3 associated with erythrocyte surface in the infected animals (P < 0.01, P < 0.05, respectively). Levels of prothrombin time and Activated Partial Thromboplastin Time were increased, while fibrinogen level was decreased (P < 0.01) in IG. CD61 microparticles were higher (P < 0.05) in IG due to platelet activation. Thus, it was established a positive correlation (P < 0.01) between platelets count and fibrinogen (Figure 3, R = 0.84, P < 0.001). Therefore, the platelet consumption component was preponderant in relation to autoimmune causes. Finally, regarding the erythrocytes, the autoimmune component played an important role, did not causing hemolytic reaction in this acute experimental time. (C) 2017 Published by Elsevier Ltd. (AU)

FAPESP's process: 15/19478-3 - Role of kinin-B2 receptor in pathogenesis and progression of familial Alzheimer's Disease: from neurogenesis and immune response to cognition
Grantee:Micheli Mainardi Pillat
Support type: Scholarships in Brazil - Post-Doctorate